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Edinburgh/Notebook/Plates
From 2008.igem.org
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'''Plate 35:''' patches of possible pSB1A2+''crtB'' transformants from Plate 30. (12.07.08)<br /> | '''Plate 35:''' patches of possible pSB1A2+''crtB'' transformants from Plate 30. (12.07.08)<br /> | ||
'''Plate 36:''' patches of possible pSB1A2+''crtI''-mut transformants from Plates 32 and 33. (12.07.08)<br /> | '''Plate 36:''' patches of possible pSB1A2+''crtI''-mut transformants from Plates 32 and 33. (12.07.08)<br /> | ||
| - | + | '''Plate 37:''' subculture of ''C. fimi'' on NA with filter paper (cellulose). (15.07.08)<br /> | |
| - | + | '''Plates 38 and 39:''' L15 transformation (pSB1A2+rbs+''dxs''). (15.07.08)<br /> | |
| - | + | '''Plates 40 and 41:''' L13 transformation (BABEL2+''glgC''-mut1,2). (15.07.08)<br /> | |
| - | + | '''Plates 42, 44 and 45:''' Patches of possible BABEL2+''glgC''-mut1,2 transformants - subcultures from plate 41. (17.07.08)<br /> | |
| - | *Plate 43:* Patches of possible | + | *Plate 43:* Patches of possible pSB1A2+rbs+''dxs'' transformants - subcultures from plate 38 or 39. (17.07.08)<br /> |
*Plates 46~47:* L16 transformation (appY+rbs PCR product with Edinbrick 1) (18/07/08) | *Plates 46~47:* L16 transformation (appY+rbs PCR product with Edinbrick 1) (18/07/08) | ||
*Plates 48~50:* _Cellulomonas fimi_ from overnight cultures made from plate 37. (24/07/08) | *Plates 48~50:* _Cellulomonas fimi_ from overnight cultures made from plate 37. (24/07/08) | ||
Revision as of 16:33, 21 August 2008
Plates 1 and 2: transformation with a BioBrick from the Registry stock: failed. (18.06.08)
Plate 3: L1 (pSB1A2+dxs) transformation, 100μl. (27.06.08)
Plate 4: L2 (pSB1A2+appY) transformation, 100μl. (27.06.08) - Failed
Plate 5: L3 (BABEL1+glgC) transformation, 100μl. (27.06.08)
Plate 6: L4 (BABEL2+glgC) transformation, 100μl. (27.06.08)
Plate 7: L1 (pSB1A2+dxs) transformation, 900μl. (27.06.08)
Plate 8: L2 (pSB1A2+appY) transformation, 900μl. (27.06.08) - Failed
Plate 9: L3 (BABEL1+glgC) transformation, 900μl. (27.06.08)
Plate 10: L4 (BABEL2+glgC) transformation, 900μl. (27.06.08)
Plate 11: possible pSB1A2+dxs transformants. (28.06.08)
Plate 12: possible Babel1+glgC transformants. (28.06.08)
Plate 13: possible Babel2+glgC transformants. (28.06.08)
Plate 14: more subcultures from plates 5 and 6. (29.06.08)
Plates 15 and 16: retransformation of religated L2. Failed again. (03.07.08)
Plate 17: Pantoea ananatis for crt PCR reactions. (05.07.08)
Plates 18 and 19: L5 (pSB1A2+appY) transformation, 100μl and 900μl. (05.07.08)
Plate 20: subculture from plate 19. (06.07.08)
Plate 21: subcultures from plate 19. (07.07.08)
Plates 22 and 23: L6 (BABEL2+glgC-mut1) transformation. No colonies. (08.07.08)
Plates 24 and 25: L7 (BABEL2+glgC-mut2) transformation. (08.07.08)
Plates 26 and 27: subcultures from plates 24 and 25 (L7). (09.07.08)
Plates 28 and 29: Cellulomonas fimi from initial stock. (11.07.08)
Plates 30 and 31: possible pSB1A2+crtB (L9) transformants. (11.07.08)
Plates 32 and 33: possible pSB1A2+crtI-mut (L10) transformants. (11.07.08)
Plate 34: possible pSB1A2+rbs+dxs clones from Nimisha's plates. (11.07.08)
Plate 35: patches of possible pSB1A2+crtB transformants from Plate 30. (12.07.08)
Plate 36: patches of possible pSB1A2+crtI-mut transformants from Plates 32 and 33. (12.07.08)
Plate 37: subculture of C. fimi on NA with filter paper (cellulose). (15.07.08)
Plates 38 and 39: L15 transformation (pSB1A2+rbs+dxs). (15.07.08)
Plates 40 and 41: L13 transformation (BABEL2+glgC-mut1,2). (15.07.08)
Plates 42, 44 and 45: Patches of possible BABEL2+glgC-mut1,2 transformants - subcultures from plate 41. (17.07.08)
- Plate 43:* Patches of possible pSB1A2+rbs+dxs transformants - subcultures from plate 38 or 39. (17.07.08)
- Plates 46~47:* L16 transformation (appY+rbs PCR product with Edinbrick 1) (18/07/08)
- Plates 48~50:* _Cellulomonas fimi_ from overnight cultures made from plate 37. (24/07/08)
- Plates 51/52:* L19 Transforations (rbs\-_dxs_\-Edinbrick) (26/07/08: CF)
- Plates 53/54:* L20 Transforations (rbs\-_appY_\-Edinbrick) (26/07/08: CF)
- Plates 55/56:* L21 Transforations (rbs\-_crtB_\-Edinbrick) (26/07/08: CF)
- Plates 57/58:* L22 Transforations (rbs\-_crtI_\-Edinbrick) (26/07/08: CF)
- Plates 59/60:* L23 Transforations (PzntA-Edinbrick) (26/07/08: CF)
- Plates 61/62:* L24 Transforations (_crtE_\-pSB1A2 - from BABEL) (26/07/08: CF)
- Plates 63/64:* Repeat transformations of L20 (28/07/08: Yan, OG)
- Plates 65/66:* Repeat transformations of L21 (28/07/08: Yan, OG)
- Plates 67/68:* glgc mutant 1&2 transformation (29.07.2008: Yan)
- Plates 69/70:* rbs+dxs, 100 and 900 µl (30.07.2008: CF)
- Plates 71/72:* crtE coding sequence in Babel2, 100 and 900 µl (30.07.2008: CF)
- Plate 73:* Subs from Plate 67 (30.07.2008: CF)
- Plate 74:* Subs from Plates 69-72 (30.07.2008: CF)
- Plates 75/76:* L26 (Edi-rbs\-_appY_) 100 microlitres/900 microlitres (31.07.2008: HX)
- Plates 77/78:* L27 (Edi-rbs\-_crtB_) 100 microlitres/900 microlitres (31.07.2008: HX)
- Plates 79/80:* L28 (Edi\-_cenA_) 100 microlitres/900 microlitres (31.07.2008: HX)
- Plates 81/82:* L29 (Edi\-_cex_) 100 microlitres/900 microlitres (31.07.2008: HX)
- Plate 83:* Subs from Plate 76 (L26: Edi-rbs+appY) (01.08.2008: OG)
- Plate 84:* Subs from Plate 77 (L27: Edi-rbs+crtB) (01.08.2008: HX)
- Plate 85:* Subs from Plate 80 (L28: Edi+cenA) (01.08.2008: AM)
- Plate 86:* Subs from Plates 81/82 (L29: Edi+cex) (01.08.2008: AM)
- Plate 89/90:* L30 (Self-ligation of P15) 100ml/900ml (06.08.2008: HX)
- Plate 91/92:* L31 (Double digestion of P36/P39 by OG) 100ml/900ml (06.08.2008: HX)
- Plate 93:* Subculture from Plate 56 (M67, pSB1A2-rbs\-_crtB_) (05.08.08: CF)
- Plate 94:* Subculture from Plate 89,90 (07.08.08: HX)
- Plate 95:* Subculture from Plate 92 (07.08.08: HX)
- Plate 96:* LB, ampicillin plate spread with subcultures from plate 43. (HX, 07.08.08)
- Plate 97:* LB, ampicillin, 2% glucose plate spread with subcultures from plate 43. (HX, 07.08.08)
- Plates 98/99:* Transformation of L32 (_crtB_/_crtI_) (08.08.08: HX)
- Plate 100/101:* Repeat transformation of L31 (Double digestion of P36/P39 by OG) (08.08.08: HX)
- Plate 102:* Patches from plate 99 (L32, crtBI) (10.08.08: CF)
- Plate 103:* Patches from plate 101 (L31, glgC double mutant+pSB1A2) (10.08.08: CF)
- Plate 104/105:* Transform L36 pSB1A2-crtY 100µl/900µl (10.08.08: CF)
- Plate 106/107:* Transform L37 pSB1A2-rbs-crtY 100µl/900µl (10.08.08: CF)
- Plate 108:* Subcultures from plates 105 and 106 (11.08.08: CF)
- Plate 109/110:* Transformation of L33 pSB1A2-PcstA 100µl/900µl (11.08.08: Yan)
- Plate 111/112:* Transformation of L34 dxs-crtE 100µl/900µl (11.08.08: Yan)
- Plate 113/114:* Transformation of L35 dxs-lims 100µl/900µl (11.08.08: Yan)
- Plate 115:* Subs from L33 (Pl. 109/110: pSB1A2-PcstA), L34 (Pl. 111/112: dxs-crtE) and L35 (Pl. 113/114: dxs-lims)
- Plates 116/117:* Transformation of L38 (psb1A2\-_crtE_) (13.08.08: HX)
- Plate 118:* Subs from plate 116 (pSB1A2+crtE 100 microlitres). (14.08.08: AH)
- Plate 119/120:* Transformation of L40 (CenA+Edinbrick1) (14.08.08: YAN)
- Plate 121/122:* Transformation of L39 (Cex+Edinbrick1) (14.08.08: YAN)
- Plate 123/124:* Repeat to transform with L39 (cex+Edinbrick1) (15.08.08: HX)
- Plate 125/126:* Transform with L40 (cenA+Edinbrick1) (15.08.08: HX)
- Plate 127/128:* Transform with L41 (dxs/crtE) (15.08.08: HX)
- Plate 129:* CenA & CeX white colonies from Plates 120 & 123-126 (16/08: CF)
- Plate 130:* dxs-CrtE white colonies from plate 127-128 (16/08: CF)
- Plate 131/132:* maize pENTR221 transformation, ISA1 Kan 10 (16/08: CF)
- Plate 133/134:* maize pENTR221 transformation, ISA2 Kan 10 (16/08: CF)
- Plate 135:* patches(colonies) from plates 131,133 (16/08: CF)
- Plate 136/137:* Transformation with L48 (dxs+Lims+Appy) (20.08.08: Yan)
- Plate 138/139:* Transformation with L49 (CrtBI+Appy) (20.08.08: YAN)
- Plate 140/141:* Transformation with Edinbrick1 only as control (20.08.08: YAN)
