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Team:Hawaii/Notebook/2008-08-21
From 2008.igem.org
(Difference between revisions)
(New page: {{Team:Hawaii/Header}} = Things we did today = == Wetlab work == ===Construction of p+r, r+g, g+t=== :<strong> Grace</strong> :* Annealed oligonucleotides: ::* plac (I14032) for front li...) |
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::* Placed in 37C water bath for 1 min, vortexed lightly, spun down before adding ligase | ::* Placed in 37C water bath for 1 min, vortexed lightly, spun down before adding ligase | ||
:* Transformed DB3.1 cells using 5 μl ligation reaction | :* Transformed DB3.1 cells using 5 μl ligation reaction | ||
| + | |||
| + | ===BB-pRL1383a MCS re-replacement=== | ||
| + | :<strong>Grace</strong> | ||
| + | |||
| + | :* RE digested BB-pRL1383a and J33207 with XbaI and PstI; incubated overnight | ||
= Discussion = | = Discussion = | ||
Revision as of 00:57, 22 August 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Construction of p+r, r+g, g+t
- Grace
- Annealed oligonucleotides:
- plac (I14032) for front ligation
- rbs 1.0 (B0034) for back ligation
- rbs 0.6 (B0030) for front ligation
- tt (B1006) for back ligation
- Ligated:
- nir (SP cut) + B0034
- plac + B0034 (EX cut)
- B0030 + E0040 (EX cut)
- E0040 (SP cut) + B1006
- Placed in 37C water bath for 1 min, vortexed lightly, spun down before adding ligase
- Transformed DB3.1 cells using 5 μl ligation reaction
BB-pRL1383a MCS re-replacement
- Grace
- RE digested BB-pRL1383a and J33207 with XbaI and PstI; incubated overnight
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
