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Team:Hawaii/Notebook/2008-08-22
From 2008.igem.org
(Difference between revisions)
(→Overnight RE digest) |
(→Verification of transformants) |
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:* Colony PCR of transformants | :* Colony PCR of transformants | ||
:* Ran PCR reaction on 2% agarose gel | :* Ran PCR reaction on 2% agarose gel | ||
| + | ::* Can't tell 15bp difference between GFP and rbs+GFP | ||
| + | :::* Restreaked single colony and inoculated for plasmid prep to confirm | ||
| + | ::* nir+rbs colonies #11-19 appear promising (larger than nir) | ||
| + | :::* Restreaked colony #19 and inoculated for plasmid prep to confirm | ||
===Plasmid prep of J33207=== | ===Plasmid prep of J33207=== | ||
Revision as of 00:42, 23 August 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Verification of transformants
- Grace
| Construct | Colony forming units |
|---|---|
| nir+rbs | 187 |
| plac+rbs | 0 |
| rbs+GFP | 1 |
| GFP+tt | 0 |
- Colony PCR of transformants
- Ran PCR reaction on 2% agarose gel
- Can't tell 15bp difference between GFP and rbs+GFP
- Restreaked single colony and inoculated for plasmid prep to confirm
- nir+rbs colonies #11-19 appear promising (larger than nir)
- Restreaked colony #19 and inoculated for plasmid prep to confirm
Plasmid prep of J33207
- Grace
Overnight RE digest
- Grace
- BB-pRL1383a and J33207 with XbaI and PstI
- pSB1A2 with EcoRI and PstI
- GFP, GFPf, nir with EcoRI and SpeI
Made LB+amp100 plates
- Grace
Drylab Work
Name of Task
- name of person/people who performed the task
- Summary of task and what was done. Link to experiment for detailed notes if necessary.
- e.g. read through papers, worked on proposal, etc.
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
