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Team:Hawaii/Notebook/2008-08-23
From 2008.igem.org
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[[Image:082308REdigests.jpg|right|thumb|250px|EtBr stained 2% agarose gel ran at 60V for 3 hours. Thirty microliters of RE digest reaction were loaded into each well.]] | [[Image:082308REdigests.jpg|right|thumb|250px|EtBr stained 2% agarose gel ran at 60V for 3 hours. Thirty microliters of RE digest reaction were loaded into each well.]] | ||
:* Gel purified RE digests | :* Gel purified RE digests | ||
| + | ::* nir band did not show up on gel | ||
:* Ligated with pSB1A2: | :* Ligated with pSB1A2: | ||
| - | |||
::* plac+rbs | ::* plac+rbs | ||
| - | |||
::* GFP+tt | ::* GFP+tt | ||
::* GFPf+tt | ::* GFPf+tt | ||
Revision as of 01:41, 24 August 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Verification of transformants (cont.)
- Grace
- Plasmid prep of nir+rbs, rbs+GFP
| Plasmid prep | DNA concentration | 260/280 | 260/230 |
|---|---|---|---|
| nir+rbs | 394.63 ng/μl | 2.04 | 2.36 |
| rbs+GFP | 94.9 ng/μl | 2.06 | 2.17 |
3A assembly
- Grace
File:082308REdigests.jpg
EtBr stained 2% agarose gel ran at 60V for 3 hours. Thirty microliters of RE digest reaction were loaded into each well.
- Gel purified RE digests
- nir band did not show up on gel
- Ligated with pSB1A2:
- plac+rbs
- GFP+tt
- GFPf+tt
- Transformed DB3.1 cells with 5μl ligation reaction
BB-pRL1383a MCS re-replacement
- Grace
- Gel purified RE digests
- Ligated J33207 with BB-pRL1383a
- Transformed DH5&alpha cells with 5 μl ligation reaction
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
