Imperial College/22 August 2008
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===''B.subtilis''=== | ===''B.subtilis''=== | ||
*The transformation protocol 1, [http://openwetware.org/wiki/IGEM:IMPERIAL/2008/Prototype/Wetlab/Transformation_protocol_2| click here for link to the protocol], was checked today. The plate containing ''B.subtilis'' transformed with no DNA had no colonies, ''B.subtilis'' transformed with 40ng DNA contained ~10 colonies and ''B.subtilis'' transformed with 400ng DNA contained ~10 colonies. | *The transformation protocol 1, [http://openwetware.org/wiki/IGEM:IMPERIAL/2008/Prototype/Wetlab/Transformation_protocol_2| click here for link to the protocol], was checked today. The plate containing ''B.subtilis'' transformed with no DNA had no colonies, ''B.subtilis'' transformed with 40ng DNA contained ~10 colonies and ''B.subtilis'' transformed with 400ng DNA contained ~10 colonies. | ||
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+ | ===Cloning=== | ||
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+ | *Odd double digest results from the 21st checked again. Gel was visualised early (but not an image not recorded) to allow identification of the small Termiantor insert | ||
+ | *Insert was succesfully identified on the gel | ||
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+ | **All Registry sequences that were succesfully cloned appear to be ok for use |
Revision as of 19:14, 25 August 2008
20 August 2008Wet LabB.subtilis
Cloning
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