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Edinburgh/29 July 2008
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(New page: <html> <head> <title>Edinburgh iGEM 2008</title> <script type="text/javascript"> //Drop Down Tabs Menu- Author: Dynamic Drive (http://www.dynamicdrive.com) //Created: May 16th, 07' var ta...) |
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| - | * Transformation of ''glgC''-mut1,2 | + | </html> |
| - | * Cut out putative ''cenA'' and ''cex'' (both ~1.5kb) fragments from '''gel 27''' (repeat of Gel 26 but stained with SYBRsafe). Purified DNA from gel fragments | + | |
| - | * Digests of M49 | + | |
| - | * Minipreps of pSB1A2+pZntA ('''M55 | + | {|style="font color="#ffffff"; "background-color:"#cd0000"; cellpadding="0" cellspacing="4" border="4" bordercolor="#000"; border-spacing:0px; text-align:"center" width="250px" |
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| - | ** Double digests of M55 | + | |
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| + | |align="left" width="150pt"|{{#calendar: title=Edinburgh |year=2008 | month=06}} | ||
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| + | |align="left" width="150pt"|{{#calendar: title=Edinburgh |year=2008 | month=07}} | ||
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| + | |align="left" width="150pt"|{{#calendar: title=Edinburgh |year=2008 | month=08}} | ||
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| + | :::: '''[[Edinburgh/28_July_2008|< Previous Entry]]''' | ||
| + | |||
| + | == Week 7 == | ||
| + | === Tuesday 29 July 08 === | ||
| + | |||
| + | * Transformations of L20 (pSB1A2+rbs+''appY'') and L21 (pSB1A2+rbs+''crtB''), plates 63-66, failed. | ||
| + | * Transformation of L25 (pSB1A2+''glgC''-mut1,2) to '''plates 67-68''' (Yan) | ||
| + | * Cut out putative ''cenA'' and ''cex'' (both ~1.5kb) fragments from '''gel 27''' (repeat of Gel 26 but stained with SYBRsafe). Purified DNA from gel fragments. (AM) | ||
| + | * Digests of M49-M54 (pSB1A2+rbs+''crtI'') with EcoRI alone. This should generate a single 4 kb band (doing an EcoRI/PstI double digest would give a vector and insert band both around 2 kb, which would probably run at the same place, making it hard to tell whether there was an insert). These were run on '''Gel 28'''. All showed a single band at the expected place. (CF) | ||
| + | * Minipreps of putative pSB1A2+pZntA ('''M55-M60''') from L23 and putative pSB1A2+rbs+''crtE'' ('''M61-M66''') from L22. (Yan, Nimisha) | ||
| + | ** Double digests of M55-M66 with EcoRI/PstI. This should generate the following bands: ~2.2 kb for pZntA (M55-M60), ~1.1 kb for rbs+''crtE'' (M61 to M66), and ~2 kb for the pSB1A2 vector. These were run on '''Gel 29'''. M55-M60 unsuccessful; M61-M66 showed a band at the expected location for rbs+''crtE'' but seemed to indicate that L22 was still ligated into BABEL2 instead of pSB1A2. (Yan, AM)<br /> | ||
| + | <br /> | ||
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| + | :::: '''[[Edinburgh/30_July_2008|Next Entry >]]''' | ||
Revision as of 15:19, 26 August 2008
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Week 7
Tuesday 29 July 08
- Transformations of L20 (pSB1A2+rbs+appY) and L21 (pSB1A2+rbs+crtB), plates 63-66, failed.
- Transformation of L25 (pSB1A2+glgC-mut1,2) to plates 67-68 (Yan)
- Cut out putative cenA and cex (both ~1.5kb) fragments from gel 27 (repeat of Gel 26 but stained with SYBRsafe). Purified DNA from gel fragments. (AM)
- Digests of M49-M54 (pSB1A2+rbs+crtI) with EcoRI alone. This should generate a single 4 kb band (doing an EcoRI/PstI double digest would give a vector and insert band both around 2 kb, which would probably run at the same place, making it hard to tell whether there was an insert). These were run on Gel 28. All showed a single band at the expected place. (CF)
- Minipreps of putative pSB1A2+pZntA (M55-M60) from L23 and putative pSB1A2+rbs+crtE (M61-M66) from L22. (Yan, Nimisha)
- Double digests of M55-M66 with EcoRI/PstI. This should generate the following bands: ~2.2 kb for pZntA (M55-M60), ~1.1 kb for rbs+crtE (M61 to M66), and ~2 kb for the pSB1A2 vector. These were run on Gel 29. M55-M60 unsuccessful; M61-M66 showed a band at the expected location for rbs+crtE but seemed to indicate that L22 was still ligated into BABEL2 instead of pSB1A2. (Yan, AM)
- Double digests of M55-M66 with EcoRI/PstI. This should generate the following bands: ~2.2 kb for pZntA (M55-M60), ~1.1 kb for rbs+crtE (M61 to M66), and ~2 kb for the pSB1A2 vector. These were run on Gel 29. M55-M60 unsuccessful; M61-M66 showed a band at the expected location for rbs+crtE but seemed to indicate that L22 was still ligated into BABEL2 instead of pSB1A2. (Yan, AM)
