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Team:Chiba/Calendar-Home/20 August 2008
From 2008.igem.org
(Difference between revisions)
| Line 1: | Line 1: | ||
'''OUTPUT TEAM''' | '''OUTPUT TEAM''' | ||
| - | + | <br>Transformation | |
| - | Luciferase(J52008:2007,2008 I712019:2008) | + | <br>Luciferase(J52008:2007,2008 I712019:2008)(Amp) |
| + | <br>Lac Z(J33202:2007,2008)(Amp) | ||
| + | <br>Venus(E2030:2007,2008)(Kam) | ||
| + | <br>Craig Venus(Amp?Kan?) | ||
| + | <br>T9002(Amp) | ||
| + | <br>F2620(Amp) | ||
| - | + | <br>luciferase,LacZ,Venus | |
| + | <br>competent cell 50μl | ||
| + | <br>DNA 1μl | ||
| + | <br>soc 200μl | ||
| + | <br>plating 250μl | ||
| + | |||
| + | <br>Craig Venus,T9002,F2620 | ||
| + | <br>competent cell 30μl | ||
| + | <br>DNA 1μl | ||
| + | <br>soc 120μl | ||
| + | <br>plating 20μl | ||
| + | |||
| + | <br>result | ||
| + | :成功→Luciferase(J52008:2007),Lac Z(J33202:2007),Craig Venus,T9002,F2620 | ||
:失敗→Luciferase(J52008:2008 J52008:2008),Lac Z(J33202:2008),Venus(J32007:2007,2008) | :失敗→Luciferase(J52008:2008 J52008:2008),Lac Z(J33202:2008),Venus(J32007:2007,2008) | ||
Revision as of 02:19, 27 August 2008
OUTPUT TEAM
Transformation
Luciferase(J52008:2007,2008 I712019:2008)(Amp)
Lac Z(J33202:2007,2008)(Amp)
Venus(E2030:2007,2008)(Kam)
Craig Venus(Amp?Kan?)
T9002(Amp)
F2620(Amp)
luciferase,LacZ,Venus
competent cell 50μl
DNA 1μl
soc 200μl
plating 250μl
Craig Venus,T9002,F2620
competent cell 30μl
DNA 1μl
soc 120μl
plating 20μl
result
- 成功→Luciferase(J52008:2007),Lac Z(J33202:2007),Craig Venus,T9002,F2620
- 失敗→Luciferase(J52008:2008 J52008:2008),Lac Z(J33202:2008),Venus(J32007:2007,2008)
