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Team:Hawaii/Notebook/2008-06- 3
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(New page: {{Team:Hawaii/Header}} == Things we did today == * Thaw test for PCC6803 and DH5-α * Made LB and LB + amp media * Made 1000X amp stock solution, stored in 4C freezer * Tested compe...)
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(New page: {{Team:Hawaii/Header}} == Things we did today == * Thaw test for PCC6803 and DH5-α * Made LB and LB + amp media * Made 1000X amp stock solution, stored in 4C freezer * Tested compe...)
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Revision as of 04:58, 4 June 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
- Thaw test for PCC6803 and DH5-α
- Made LB and LB + amp media
- Made 1000X amp stock solution, stored in 4C freezer
- Tested competency of DH5-α (see experiment)
- Counted colonies to determine number of cells grown to OD600 = 0.3
- Cells were cryoperserved, OD600 = 0.0748
- 10 μl were plated on each plate
| Dilution | Colony Forming Units on Plate | Number of Cells per mL |
|---|---|---|
| 1 | Too many to count | n/a |
| 1/10 | Too many to count | n/a |
| 1/100 | Too many to count | n/a |
| 1/1000 | 190 | 19,000,000 |
| 1/10,000 | 40 | 4,000,000 |
| 1/100,000 | 23 | 23,000,000 |
| 1/1,000,000 | 6 | 60,000 |
Discussion
We have lots of cells (yay!). Cryofreezing will result in the loss cell population, but we will very likely still have viable cells after thawing.
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
