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User:University of Washington/27 August 2008
From 2008.igem.org
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==LuxR from AraC and TetR(Faifan)== | ==LuxR from AraC and TetR(Faifan)== | ||
| - | - | + | <h4>3A ligation</h4> |
| + | |||
| + | -miniprepped x 6 | ||
| + | |||
| + | -submit forward sequencing x 6 | ||
| + | |||
| + | <h4>with CIP reaction</h4> | ||
| + | |||
| + | - ran gel on digested promoter, GFP, and LuxR and cut bands(3184 bp for promoter vector, 898 bp for GFP insert, and 958 bp for LuxR insert) | ||
| + | |||
| + | - did gel purification | ||
| + | |||
| + | - nanodropped at 260 nm | ||
| + | |||
| + | |||
---- | ---- | ||
Back to [[Team:University_of_Washington/Notebook#Notebook]] | Back to [[Team:University_of_Washington/Notebook#Notebook]] | ||
Revision as of 23:14, 27 August 2008
LuxR from AraC and TetR(Faifan)
3A ligation
-miniprepped x 6
-submit forward sequencing x 6
with CIP reaction
- ran gel on digested promoter, GFP, and LuxR and cut bands(3184 bp for promoter vector, 898 bp for GFP insert, and 958 bp for LuxR insert)
- did gel purification
- nanodropped at 260 nm
Back to Team:University_of_Washington/Notebook#Notebook
