Team:Hawaii/Notebook/2008-08-27

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(Difference between revisions)
(→Construction of p+r)
(→Construction of p+r)
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:* Ran RE digested products on gel
:* Ran RE digested products on gel
 +
::* nir had three bands. Band at ~730bp = ?
 +
::* BB-pRL1383a -- still couldn't see cut out of GFP. Assumed double digest successful, too little DNA to see GFP.
 +
::* J33207 wrong size
:* Extracted nir, C0012 vector, BB-pRL1383a from gel
:* Extracted nir, C0012 vector, BB-pRL1383a from gel
:* 3A assembly (ligation) of C0012 vector (pSB1A2) with:
:* 3A assembly (ligation) of C0012 vector (pSB1A2) with:

Revision as of 23:16, 27 August 2008

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Things we did today

Wetlab work

Construction of p+r

EtBr stained 2% agarose gel ran at 60V for 2.25 hours. Thirty microliters of RE digested DNA were loaded into each well.
Grace
  • Ran RE digested products on gel
  • nir had three bands. Band at ~730bp = ?
  • BB-pRL1383a -- still couldn't see cut out of GFP. Assumed double digest successful, too little DNA to see GFP.
  • J33207 wrong size
  • Extracted nir, C0012 vector, BB-pRL1383a from gel
  • 3A assembly (ligation) of C0012 vector (pSB1A2) with:
  • nir and rbs (B0034)
  • plac and rbs(B0034)
  • pSB1A2 (negative control)
  • Transformed DB3.1 cells using 5 μl ligation reaction

Discussion

Quote of the Day

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson


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