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Edinburgh/5 August 2008
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(New page: <html> <head> <title>Edinburgh iGEM 2008</title> <script type="text/javascript"> //Drop Down Tabs Menu- Author: Dynamic Drive (http://www.dynamicdrive.com) //Created: May 16th, 07' var ta...) |
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<li><a href="https://2008.igem.org/Team:Edinburgh" title="Home"><span>Home</span></a></li> | <li><a href="https://2008.igem.org/Team:Edinburgh" title="Home"><span>Home</span></a></li> | ||
<li><a href="https://2008.igem.org/Team:Edinburgh/Project" title="Project" rel="dropmenu1_a"><span>The Project</span></a></li> | <li><a href="https://2008.igem.org/Team:Edinburgh/Project" title="Project" rel="dropmenu1_a"><span>The Project</span></a></li> | ||
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<li><a href="https://2008.igem.org/Team:Edinburgh/Modeling" title="Modelling" rel="dropmenu2_a"><span>Modelling</span></a></li> | <li><a href="https://2008.igem.org/Team:Edinburgh/Modeling" title="Modelling" rel="dropmenu2_a"><span>Modelling</span></a></li> | ||
| - | <li><a href="https://2008.igem.org/Team:Edinburgh/Notebook" title="Notebook"><span>Notebook</span></a></li> | + | <li><a href="https://2008.igem.org/Team:Edinburgh/Notebook" title="Notebook"><span>Notebook</span></a></li> |
| + | <li><a href="https://2008.igem.org/Team:Edinburgh/Results" title="Results" rel="dropmenu1_a"><span>Results</span></a></li> | ||
| + | <li><a href="http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2008&group=Edinburgh" title="Parts" rel="dropmenu1_a"><span>BioBrick Parts</span></a></li> | ||
| + | <li><a href="https://2008.igem.org/Team:Edinburgh/Team" title="Team" ><span>The Team</span></a></li> | ||
</ul> | </ul> | ||
</div> | </div> | ||
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| - | === | + | <table> |
| - | ==== Tuesday 5 August 08 | + | <html> |
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| + | {|style="font color="#ffffff"; "background-color:"#cd0000"; cellpadding="0" cellspacing="4" border="4" bordercolor="#000"; border-spacing:0px; text-align:"center" width="250px" | ||
| + | </table> | ||
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| + | {| align="left" | ||
| + | |- | ||
| + | |align="left" width="150pt"|{{#calendar: title=Edinburgh |year=2008 | month=06}} | ||
| + | |- | ||
| + | |align="left" width="150pt"|{{#calendar: title=Edinburgh |year=2008 | month=07}} | ||
| + | |- | ||
| + | |align="left" width="150pt"|{{#calendar: title=Edinburgh |year=2008 | month=08}} | ||
| + | |} | ||
| + | |||
| + | :::: '''[[Edinburgh/4_August_2008|< Previous Entry]]''' | ||
| + | |||
| + | == Week 8 == | ||
| + | === Tuesday 5 August 08 === | ||
* M72 (pSB1A2+rbs+''dxs''), M79 (BABEL2+''crtE'') and M82 (pSB1A2+rbs+''appY'') submitted for seqencing. (CF) | * M72 (pSB1A2+rbs+''dxs''), M79 (BABEL2+''crtE'') and M82 (pSB1A2+rbs+''appY'') submitted for seqencing. (CF) | ||
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* M68-69 (''glgc''-mut1,2 in pSB1A2) and M92 (pSB1A2+''cex'') were digested with a) PstI and b) EcoRI/PstI, but gel leaked. Entire process needs repeating tomorrow! (CF, OG) | * M68-69 (''glgc''-mut1,2 in pSB1A2) and M92 (pSB1A2+''cex'') were digested with a) PstI and b) EcoRI/PstI, but gel leaked. Entire process needs repeating tomorrow! (CF, OG) | ||
* Digested P36 (''glgC''-mut1,2) with Edinbrick1 using Spe1/EcoR1 - to be continued tomorrow. (OG) | * Digested P36 (''glgC''-mut1,2) with Edinbrick1 using Spe1/EcoR1 - to be continued tomorrow. (OG) | ||
| - | * '''Plate 93''' created from subculture of plate 56 (M67, pSB1A2+rbs+''crtB'') | + | * '''Plate 93''' created from subculture of plate 56 (M67, pSB1A2+rbs+''crtB'')<br /> |
| + | <br /> | ||
| + | |||
| + | :::: '''[[Edinburgh/6_August_2008|Next Entry >]]''' | ||
Revision as of 10:20, 28 August 2008
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Week 8
Tuesday 5 August 08
- M72 (pSB1A2+rbs+dxs), M79 (BABEL2+crtE) and M82 (pSB1A2+rbs+appY) submitted for seqencing. (CF)
- Minipreps M97-104 of pSB1A2 cenA (2, 7-13) and M105-108 Minipreps M105-108 of pSB1A2+cex. (Yan)
- M97-108 were double-digested with EcoRI/PstI and run on gel 37. (Yan)
- Purification and self-ligation of P50 (3rd mutagenesis of glgc-mut1,2) (L30). (HX)
- M68-69 (glgc-mut1,2 in pSB1A2) and M92 (pSB1A2+cex) were digested with a) PstI and b) EcoRI/PstI, but gel leaked. Entire process needs repeating tomorrow! (CF, OG)
- Digested P36 (glgC-mut1,2) with Edinbrick1 using Spe1/EcoR1 - to be continued tomorrow. (OG)
- Plate 93 created from subculture of plate 56 (M67, pSB1A2+rbs+crtB)
