Team:Hawaii/Notebook/2008-06- 3

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[[Image:DSC00696.JPG|left|thumb|250px|DH5-α dilutions plated on LB]]
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[[Image:DSC00696.JPG|center|thumb|600px|DH5-α dilutions plated on LB]]
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== Discussion ==
== Discussion ==

Revision as of 07:11, 4 June 2008

Projects Events Resources
Sponsors Experiments Milestones Protocols
Notebook (t) Meetings (t)

Things we did today

  • Thaw test for PCC6803 and DH5-α
  • Made LB and LB + amp media
  • Made 1000X amp stock solution, stored in 4C freezer
  • Tested competency of DH5-α (see experiment)
  • Counted colonies to determine number of cells grown to OD600 = 0.3
  • Cells were cryoperserved, OD600 = 0.0748
  • 10 μl were plated on each plate
Cell Count
Dilution Colony Forming Units on Plate Number of Cells per mL
1 Too many to count n/a
1/10 Too many to count n/a
1/100 Too many to count n/a
1/1000 190 19,000,000
1/10,000 40 4,000,000
1/100,000 23 23,000,000
1/1,000,000 6 60,000
DH5-α dilutions plated on LB

Discussion

We have lots of cells (yay!). Cryofreezing will result in the loss cell population, but we will very likely still have viable cells after thawing.


[http://manoa.hawaii.edu/ Sponsor_UHM.gif][http://manoa.hawaii.edu/ovcrge/ Sponsor_OVCRGE.gif][http://www.ctahr.hawaii.edu Sponsor_CTAHR.gif]

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