Edinburgh/24 July 2008

From 2008.igem.org

(Difference between revisions)
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<html>
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<div id="header">{{Template:Team:Edinburgh/Templates/notebook-entry/header}}</div>
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<head>
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<title>Edinburgh iGEM 2008</title>
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<script type="text/javascript">
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//Drop Down Tabs Menu- Author: Dynamic Drive (http://www.dynamicdrive.com)
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//Created: May 16th, 07'
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//No need to edit beyond here////////////////////////
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this.shimobject.style.display='none'
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</script>
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<style>
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.ddcolortabs{
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padding: 0;
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width: 100%;
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background: transparent;
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voice-family: "\"}\"";
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.ddcolortabs ul{
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text-transform:uppercase;
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.ddcolortabs a{
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float:left;
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color: white;
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background: black url(media/color_tabs_left.gif) no-repeat left top;
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margin:0 2px 0 0;
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padding:0 0 1px 3px;
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text-decoration:none;
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letter-spacing: 1px;
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.ddcolortabs a span{
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float:left;
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display:block;
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background: transparent url(media/color_tabs_right.gif) no-repeat right top;
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padding: 4px 8px 2px 7px;
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.ddcolortabs a span{
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float:none;
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.ddcolortabs a:hover{
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background-color: #591f20;
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.ddcolortabs a:hover span{
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background-color: #591f20;
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.ddcolortabs .selected a, #ddcolortabs .selected a span{ /*currently selected tab*/
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background-color: #591f20;
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}
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.ddcolortabsline{
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clear: both;
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padding: 0;
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width: 100%;
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height: 8px;
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line-height: 8px;
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background: black;
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border-top: 1px solid #fff; /*Remove this to remove border between bar and tabs*/
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}
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/* ######### Style for Drop Down Menu ######### */
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.dropmenudiv_a{
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position:absolute;
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top: 0;
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border: 1px solid black; /*THEME CHANGE HERE*/
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border-top-width: 8px; /*Top border width. Should match height of .ddcolortabsline above*/
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border-bottom-width: 0;
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font:normal 12px Arial;
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line-height:18px;
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z-index:100;
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background-color: white;
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width: 200px;
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.dropmenudiv_a a{
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width: auto;
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display: block;
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text-indent: 5px;
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border-top: 0 solid #678b3f;
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border-bottom: 1px solid #678b3f; /*THEME CHANGE HERE*/
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padding: 2px 0;
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text-decoration: none;
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color: black;
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* html .dropmenudiv_a a{ /*IE only hack*/
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width: 100%;
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.dropmenudiv_a a:hover{ /*THEME CHANGE HERE*/
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background-color: #8a3c3d;
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color: white;
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</style>
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</head>
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<!-- CSS for Drop Down Tabs Menu #1 -->
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<link rel="stylesheet" type="text/css" href="ddcolortabs.css" />
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<div id="colortab" class="ddcolortabs">
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<ul>
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<li><a href="https://2008.igem.org/Team:Edinburgh" title="Home"><span>Home</span></a></li>
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<li><a href="https://2008.igem.org/Team:Edinburgh/Project" title="Project" rel="dropmenu1_a"><span>The Project</span></a></li>
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<li><a href="https://2008.igem.org/Team:Edinburgh/Modeling" title="Modelling" rel="dropmenu2_a"><span>Modelling</span></a></li>
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<li><a href="https://2008.igem.org/Team:Edinburgh/Notebook" title="Notebook"><span>Notebook</span></a></li>
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<li><a href="https://2008.igem.org/Team:Edinburgh/Results" title="Results" rel="dropmenu1_a"><span>Results</span></a></li>
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<li><a href="http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2008&group=Edinburgh" title="Parts" rel="dropmenu1_a"><span>BioBrick Parts</span></a></li>
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<li><a href="https://2008.igem.org/Team:Edinburgh/Team" title="Team" ><span>The Team</span></a></li>
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</ul>
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</div>
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<div class="ddcolortabsline">&nbsp;</div>
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<div id="dropmenu1_a" class="dropmenudiv_a">
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<a href="https://2008.igem.org/Team:Edinburgh/Team">Overview</a>
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<a href="https://2008.igem.org/Team:Edinburgh/Team">Step1</a>
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<a href="https://2008.igem.org/Team:Edinburgh/Team">Step2</a>
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</div>
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tabdropdown.init("colortab", 3)
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<table>
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<html>
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<style type="text/css">
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table.calendar          { margin: 0; padding: 2px; }
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table.calendar td      { margin: 0; padding: 1px; vertical-align: top; }
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table.month .heading td { padding:1px; background-color: black; color: white; text-align:center; font-size:140%; font-weight:bold; }
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table.month td.today    { background-color:#cd0000; }
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    font-weight: bold;
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#bodyContent table.month a { background:none; padding:0 }
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.day-active { color:#cd0000 }
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.day-empty  { color:#000000 }
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</style>
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</html>
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{|style="font color="#ffffff"; "background-color:"#cd0000"; cellpadding="0" cellspacing="4" border="4" bordercolor="#000"; border-spacing:0px; text-align:"center" width="250px"
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</table>
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{| align="left"
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|-
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|align="left" width="150pt"|{{#calendar: title=Edinburgh |year=2008 | month=06}}
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|-
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|align="left" width="150pt"|{{#calendar: title=Edinburgh |year=2008 | month=07}}
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|-
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|align="left" width="150pt"|{{#calendar: title=Edinburgh |year=2008 | month=08}}
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|}
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:::: '''[[Edinburgh/23_July_2008|< Previous Entry]]'''
:::: '''[[Edinburgh/23_July_2008|< Previous Entry]]'''

Revision as of 14:10, 28 August 2008

< Previous Entry

Week 6

Thursday 24 July 08

  • Purified PCR products P28 (rbs+crtB) and P29 (rbs+crtI). (OG)
  • Double digestions using XbaI/PstI (buffer H) to add P12 (rbs+dxs), P15 (rbs+appY), P28 (rbs+crtB) and P29 (rbs+crtI) to Edinbrick1. (Yan, OG)
  • Double digestion using SacI/SpeI (BufferE) to add P31 (pZntA) to Edinbrick1. (Yan, OG)
  • Purified genomic DNA from C. fimi culture (made from plate 37, incubated in 25ml bottle at 30C for 48 hours with shaking). Used Promega Wizard Genomic DNA Purification Kit, lysozyme and RNAse+EDTA (as replacement for proprietary RNAse Solution, of which none was left). Final solution left overnight in refrigerator at 4°C. (AM)
  • Plated C. fimi culture from plate 37...
    • Incubated in 25ml bottle for 48 hours at 30°C with shaking ... to Plate 48.
    • Incubated in 50ml bottle for 24 hours at 30°C with shaking ... to Plate 49.
    • Incubated in 250ml flask for 24 hours at 30°C with shaking ... to Plate 50. (AM)
  • PCR of cenA (P32), cenB (P33), cenC (P34), cex (P35), annealing 1 min 55°C, extension 90 sec 70°C. Run on Gel 24 - unsuccessful. (AM)


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