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User:University of Washington/29 August 2008
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| + | ==LuxR from AraC and TetR(Faifan)== | ||
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| + | <h4>3A assembly</h4> | ||
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| + | - sequencing result came back. One out of three GPF constructs showed successful ligation!!! (culture#3) None of the LuxR constructs worked. [Re: pick more colonies from LuxR plate, stock GFP plasmid and shock miniprep into MG1655Z1] | ||
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| + | - streaked out MG1655Z1 from stock onto Tsy plate. | ||
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| + | <h4>ligation with CIP reaction</h4> | ||
| + | |||
| + | -no growth in any plates. =[ It could be because the ligation wasn't set up right..may be not enough DNA or something. | ||
| + | |||
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Back to [[Team:University_of_Washington/Notebook#Notebook]] | Back to [[Team:University_of_Washington/Notebook#Notebook]] | ||
Latest revision as of 19:36, 29 August 2008
LuxR from AraC and TetR(Faifan)
3A assembly
- sequencing result came back. One out of three GPF constructs showed successful ligation!!! (culture#3) None of the LuxR constructs worked. [Re: pick more colonies from LuxR plate, stock GFP plasmid and shock miniprep into MG1655Z1]
- streaked out MG1655Z1 from stock onto Tsy plate.
ligation with CIP reaction
-no growth in any plates. =[ It could be because the ligation wasn't set up right..may be not enough DNA or something.
Back to Team:University_of_Washington/Notebook#Notebook
