Team:Chiba/jk/β

From 2008.igem.org

(Difference between revisions)
(→Week 1)
(→Week 1)
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*17 August, 2008  
*17 August, 2008  
:Transforation
:Transforation
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:*BBa_R0040(2008) -> No colonies on plates  
+
::*[[http://partsregistry.org/Part:BBa_R0040|BBa_R0040]](2008) -> No colonies on plates  
-
:*BBa_C0070(2008) -> No colonies on plates  
+
::*[[http://partsregistry.org/Part:BBa_C0070|BBa_C0070]](2008) -> No colonies on plates  
-
:*BBa_C0076(2008) -> No colonies on plates  
+
::*[[http://partsregistry.org/Part:BBa_C0076|BBa_C0076]](2008) -> No colonies on plates  
-
:*BBa_C0077(2008) -> No colonies on plates  
+
::*[[http://partsregistry.org/Part:BBa_C0077|BBa_C0077]](2008) -> No colonies on plates  
-
:*BBa_C0078(2008) -> No colonies on plates  
+
::*[[http://partsregistry.org/Part:BBa_C0078|BBa_C0078]](2008) -> No colonies on plates  
-
:*BBa_C0079(2008) -> No colonies on plates
+
::*[[http://partsregistry.org/Part:BBa_C0079|BBa_C0079]](2008) -> No colonies on plates
*18 August, 2008  
*18 August, 2008  
:Transformation
:Transformation
-
:*BBa_C0070(2007)
+
::*[[http://partsregistry.org/Part:BBa_C0070|BBa_C0070]](2007)
-
:*BBa_C0070(2006)
+
::*[[http://partsregistry.org/Part:BBa_C0070|BBa_C0070]](2006)
-
:*BBa_C0076(2007)
+
::*[[http://partsregistry.org/Part:BBa_C0076|BBa_C0076]](2007)
-
:*BBa_C0076(2006) -> No colonies on plates
+
::*[[http://partsregistry.org/Part:BBa_C0076|BBa_C0076]](2006) -> No colonies on plates
-
:*BBa_C0078(2007)
+
::*[[http://partsregistry.org/Part:BBa_C0078|BBa_C0078]](2007)
-
:*BBa_C0078(2006)
+
::*[[http://partsregistry.org/Part:BBa_C0078|BBa_C0078]](2006)
-
:*BBa_C0170(2007)
+
::*[[http://partsregistry.org/Part:BBa_C0170|BBa_C0170]](2007)
-
:*BBa_C0170(2006)
+
::*[[http://partsregistry.org/Part:BBa_C0170|BBa_C0170]](2006)
-
:*BBa_C0178(2007)
+
::*[[http://partsregistry.org/Part:BBa_C0178|BBa_C0178]](2007)
-
:*BBa_C0178(2006)
+
::*[[http://partsregistry.org/Part:BBa_C0178|BBa_C0178]](2006)
*19 August, 2008  
*19 August, 2008  

Revision as of 12:59, 30 August 2008

β班の実験ノート


Contents

Week 1

  • 17 August, 2008
Transforation


  • 18 August, 2008
Transformation
  • 19 August, 2008
PCR
①BBa_I9026(2007)
②BBa_I9026(2006)
③BBa_I9030(2007)
④BBa_I9030(2006)
⑤BBa_S03154(2007)
⑥BBa_S03154(2006)
⑦BBa_F2620(2007)
Gel Check
①～②
  • 20 August, 2008
Sigma prep
  • BBa_C0070(2007)
  • BBa_C0070(2006)
  • BBa_C0076(2006)
  • BBa_C0078(2007)
  • BBa_C0078(2006)
Digestion
  • BBa_C0170(2007)
  • BBa_C0170(2006)
  • BBa_C0178(2007)
  • BBa_C0178(2006)
Gel Check
  • 21 August, 2008
PCR
  • BBa_C0070(2007)
  • BBa_C0070(2006)
  • BBa_C0076(2006)
  • BBa_C0078(2007)
  • BBa_C0078(2006)

Week 2

  • 27 August, 2008
Colony PCR of 5 colonies from ligation plates (27/8) and one from control plate(F2620).

Week 3

reference

  • L. Steindler.V.Venturi.:Detection of quorum sensing N-acyl homoserine lactone.FEMS Microbiol Lett 266 (2007) 1-9
クオラムセンシングのクロストークについてのミニレビュー。クオラムセンシングを利用したAHLバイオセンサーについて、Table 1.にまとまっている。
  • M.K Winson et al./FEMS Microbiology Letters 163 (1998) 185-192
Figure 2.hは、LuxR、RhlR、LasRそれぞれの、C4-C14のN-acyl-L-homoserine lactone,C4-C14のN-(3-oxoacyl)-L-homoserine lactoneに対する感受性を比較したグラフ。


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