Team:Chiba/jk/β

From 2008.igem.org

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*19 August, 2008  
*19 August, 2008  
:[[Team:Chiba/protocol/PCR|PCR]]
:[[Team:Chiba/protocol/PCR|PCR]]
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::①BBa_I9026(2007)
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::①[http://partsregistry.org/Part:BBa_I9026| BBa_I9026](2007)
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::②BBa_I9026(2006)
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::②[http://partsregistry.org/Part:BBa_I9026| BBa_I9026](2006)
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::③BBa_I9030(2007)
+
::③[http://partsregistry.org/Part:BBa_I9030| BBa_I9030](2007)
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::④BBa_I9030(2006)
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::④[http://partsregistry.org/Part:BBa_I9030| BBa_I9030](2006)
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::⑤BBa_S03154(2007)
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::⑤[http://partsregistry.org/Part:BBa_S3154| BBa_S3154](2007)
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::⑥BBa_S03154(2006)
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::⑥[http://partsregistry.org/Part:BBa_S3154| BBa_S3154](2006)
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::⑦BBa_F2620(2007)
+
::⑦[http://partsregistry.org/Part:BBa_F2620| BBa_F2620](2007)･･･control
:[[Team:Chiba/protocol/gelcheck|Gel Check]]
:[[Team:Chiba/protocol/gelcheck|Gel Check]]

Revision as of 13:06, 30 August 2008

β班の実験ノート


Contents

Week 1

  • 17 August, 2008
Transforation
  • [http://partsregistry.org/Part:BBa_R0040| BBa_R0040](2008) -> No colonies on plates
  • [http://partsregistry.org/Part:BBa_C0070| BBa_C0070](2008) -> No colonies on plates
  • [http://partsregistry.org/Part:BBa_C0076| BBa_C0076](2008) -> No colonies on plates
  • [http://partsregistry.org/Part:BBa_C0077| BBa_C0077](2008) -> No colonies on plates
  • [http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2008) -> No colonies on plates
  • [http://partsregistry.org/Part:BBa_C0079| BBa_C0079](2008) -> No colonies on plates


  • 18 August, 2008
Transformation
  • [http://partsregistry.org/Part:BBa_C0070| BBa_C0070](2007)
  • [http://partsregistry.org/Part:BBa_C0070| BBa_C0070](2006)
  • [http://partsregistry.org/Part:BBa_C0076| BBa_C0076](2007)
  • [http://partsregistry.org/Part:BBa_C0076| BBa_C0076](2006) -> No colonies on plates
  • [http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2007)
  • [http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2006)
  • [http://partsregistry.org/Part:BBa_C0170| BBa_C0170](2007)
  • [http://partsregistry.org/Part:BBa_C0170| BBa_C0170](2006)
  • [http://partsregistry.org/Part:BBa_C0178| BBa_C0178](2007)
  • [http://partsregistry.org/Part:BBa_C0178| BBa_C0178](2006)
  • 19 August, 2008
PCR
①[http://partsregistry.org/Part:BBa_I9026| BBa_I9026](2007)
②[http://partsregistry.org/Part:BBa_I9026| BBa_I9026](2006)
③[http://partsregistry.org/Part:BBa_I9030| BBa_I9030](2007)
④[http://partsregistry.org/Part:BBa_I9030| BBa_I9030](2006)
⑤[http://partsregistry.org/Part:BBa_S3154| BBa_S3154](2007)
⑥[http://partsregistry.org/Part:BBa_S3154| BBa_S3154](2006)
⑦[http://partsregistry.org/Part:BBa_F2620| BBa_F2620](2007)･･･control
Gel Check
①～②
  • 20 August, 2008
Sigma prep
  • BBa_C0070(2007)
  • BBa_C0070(2006)
  • BBa_C0076(2006)
  • BBa_C0078(2007)
  • BBa_C0078(2006)
Digestion
  • BBa_C0170(2007)
  • BBa_C0170(2006)
  • BBa_C0178(2007)
  • BBa_C0178(2006)
Gel Check
  • 21 August, 2008
PCR
  • BBa_C0070(2007)
  • BBa_C0070(2006)
  • BBa_C0076(2006)
  • BBa_C0078(2007)
  • BBa_C0078(2006)

Week 2

  • 27 August, 2008
Colony PCR of 5 colonies from ligation plates (27/8) and one from control plate(F2620).

Week 3

reference

  • L. Steindler.V.Venturi.:Detection of quorum sensing N-acyl homoserine lactone.FEMS Microbiol Lett 266 (2007) 1-9
クオラムセンシングのクロストークについてのミニレビュー。クオラムセンシングを利用したAHLバイオセンサーについて、Table 1.にまとまっている。
  • M.K Winson et al./FEMS Microbiology Letters 163 (1998) 185-192
Figure 2.hは、LuxR、RhlR、LasRそれぞれの、C4-C14のN-acyl-L-homoserine lactone,C4-C14のN-(3-oxoacyl)-L-homoserine lactoneに対する感受性を比較したグラフ。


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