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Team:Chiba/jk/β
From 2008.igem.org
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| - | + | :[[Team:Chiba/protocol/transformation|Transforation]] | |
::*[http://partsregistry.org/Part:BBa_C0070| BBa_C0070](2007) | ::*[http://partsregistry.org/Part:BBa_C0070| BBa_C0070](2007) | ||
::*[http://partsregistry.org/Part:BBa_C0070| BBa_C0070](2006) | ::*[http://partsregistry.org/Part:BBa_C0070| BBa_C0070](2006) | ||
Revision as of 13:29, 30 August 2008
β班の実験ノート
Contents |
Week 1
- 17 August, 2008
- Transforation
- [http://partsregistry.org/Part:BBa_R0040| BBa_R0040](2008) -> No colonies on plates
- [http://partsregistry.org/Part:BBa_C0070| BBa_C0070](2008) -> No colonies on plates
- [http://partsregistry.org/Part:BBa_C0076| BBa_C0076](2008) -> No colonies on plates
- [http://partsregistry.org/Part:BBa_C0077| BBa_C0077](2008) -> No colonies on plates
- [http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2008) -> No colonies on plates
- [http://partsregistry.org/Part:BBa_C0079| BBa_C0079](2008) -> No colonies on plates
- 18 August, 2008
- Transforation
- [http://partsregistry.org/Part:BBa_C0070| BBa_C0070](2007)
- [http://partsregistry.org/Part:BBa_C0070| BBa_C0070](2006)
- [http://partsregistry.org/Part:BBa_C0076| BBa_C0076](2007)
- [http://partsregistry.org/Part:BBa_C0076| BBa_C0076](2006) -> No colonies on plates
- [http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2007)
- [http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2006)
- [http://partsregistry.org/Part:BBa_C0170| BBa_C0170](2007)
- [http://partsregistry.org/Part:BBa_C0170| BBa_C0170](2006)
- [http://partsregistry.org/Part:BBa_C0178| BBa_C0178](2007)
- [http://partsregistry.org/Part:BBa_C0178| BBa_C0178](2006)
- 19 August, 2008
- PCR
- [http://partsregistry.org/Part:BBa_I9026| BBa_I9026](2007)
- [http://partsregistry.org/Part:BBa_I9026| BBa_I9026](2006)
- [http://partsregistry.org/Part:BBa_I9030| BBa_I9030](2007)
- [http://partsregistry.org/Part:BBa_I9030| BBa_I9030](2006)
- [http://partsregistry.org/Part:BBa_S3154| BBa_S3154](2007)
- [http://partsregistry.org/Part:BBa_S3154| BBa_S3154](2006)
- [http://partsregistry.org/Part:BBa_F2620| BBa_F2620](2007)･･･control
- Gel Check
- [http://partsregistry.org/Part:BBa_I9026| BBa_I9026](2007)
- [http://partsregistry.org/Part:BBa_I9026| BBa_I9026](2006)
- [http://partsregistry.org/Part:BBa_I9030| BBa_I9030](2007)
- [http://partsregistry.org/Part:BBa_I9030| BBa_I9030](2006)
- [http://partsregistry.org/Part:BBa_S3154| BBa_S3154](2007)
- [http://partsregistry.org/Part:BBa_S3154| BBa_S3154](2006)
- [http://partsregistry.org/Part:BBa_F2620| BBa_F2620](2007)
- 20 August, 2008
- Mini prep
- [http://partsregistry.org/Part:BBa_C0070| BBa_C0070](2007)
- [http://partsregistry.org/Part:BBa_C0070| BBa_C0070](2006)
- [http://partsregistry.org/Part:BBa_C0076| BBa_C0076](2007)
- [http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2007)
- [http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2006)
- Digestion
- [http://partsregistry.org/Part:BBa_C0170| BBa_C0170](2007)
- [http://partsregistry.org/Part:BBa_C0170| BBa_C0170](2006)
- [http://partsregistry.org/Part:BBa_C0178| BBa_C0178](2007)
- [http://partsregistry.org/Part:BBa_C0178| BBa_C0178](2006)
- Gel Check
- [http://partsregistry.org/Part:BBa_C0070| BBa_C0070](2007)
- [http://partsregistry.org/Part:BBa_C0070| BBa_C0070](2006)
- [http://partsregistry.org/Part:BBa_C0076| BBa_C0076](2007)
- [http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2007)
- [http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2006)
- 21 August, 2008
- PCR
- [http://partsregistry.org/Part:BBa_C0070| BBa_C0070](2007)
- [http://partsregistry.org/Part:BBa_C0070| BBa_C0070](2006)
- [http://partsregistry.org/Part:BBa_C0076| BBa_C0076](2007)
- [http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2007)
- [http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2006)
- Gel Check
- [http://partsregistry.org/Part:BBa_C0070| BBa_C0070](2007)
- [http://partsregistry.org/Part:BBa_C0070| BBa_C0070](2006)
- [http://partsregistry.org/Part:BBa_C0076| BBa_C0076](2007) -> None
- [http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2007) -> None
- [http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2006) -> None
- Transforation
- (Locate & 2 Punch ~iyama ver.~)
- [http://partsregistry.org/Part:BBa_C0076| BBa_C0076](2008) -> No colonies on plates
- [http://partsregistry.org/Part:BBa_C0077| BBa_C0077](2008) -> No colonies on plates
- [http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2008) -> No colonies on plates
- 22 August, 2008
- 23 August, 2008
Week 2
- 24 August, 2008
- 25 August, 2008
- 26 August, 2008
- 27 August, 2008
- PCR
- Colony PCR of 5 colonies from ligation plates (27/8) and one from control plate([http://partsregistry.org/Part:BBa_F2620| BBa_F2620](2007)).
- 30 August, 2008
Week 3
- 31 August, 2008
- 1 September, 2008
Week 4
reference
- L. Steindler.V.Venturi.:Detection of quorum sensing N-acyl homoserine lactone.FEMS Microbiol Lett 266 (2007) 1-9
- クオラムセンシングのクロストークについてのミニレビュー。クオラムセンシングを利用したAHLバイオセンサーについて、Table 1.にまとまっている。
- M.K Winson et al.Construction and analysis of luxCDABE-based plasmid sensors
for investigating N-acyl homoserine lactone-mediated quorum sensing.FEMS Microbiology Letters 163 (1998) 185-192
- Figure 2.hは、LuxR、RhlR、LasRそれぞれの、C4-C14のN-acyl-L-homoserine lactone,C4-C14のN-(3-oxoacyl)-L-homoserine lactoneに対する感受性を比較したグラフ。
- Michiko E. Taga. Bonnie L. Bassler.:Chemical communication among bacteria.PNAS.November 25, 2003 �100 �suppl.2 �
- クオラムセンシングについての総説。アシル基の異なるAHL、およびそのシンターゼの紹介、AIPを用いたコミュニケーション、AiiA（AHL分解酵素）についてのまとめがある。
- K.M.GRAY et al.Interchangeability and Specificity of Components from the Quorum-Sensing Regulatory Systems of Vibrio fischeri and Pseudomonas aeruginosa.J.BACTERIOL.176,1994,3076-3080
- LasとLuxのクロストークについて調べた論文。Table 1とTable 2で、promotor、レセプタータンパク質、インデューサーそれぞれを交換して、レポーターの発現の様子を調べている。
- FK Balagadde et al.:A synthetic predator–prey ecosystem.Molecular Systems Biology 4:187,2008
- LuxとLasによるクオラムセンシングを利用した、プレデター・プレイモデル。LuxとLasのクロストークについて言及あり。
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