Team:Chiba/jk/β

From 2008.igem.org

(Difference between revisions)
(→Week 1)
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==Week 2==
==Week 2==
*24 August, 2008
*24 August, 2008
 +
:[[Team:Chiba/protocol/DNA Purification/sigma|Mini prep]]
 +
::*[http://partsregistry.org/Part:BBa_J13002| BBa_J13002](2007)
 +
::*[http://partsregistry.org/Part:BBa_J13002| BBa_J13002](2006)
 +
::*[http://partsregistry.org/Part:BBa_J04500| BBa_J04500](2007)
 +
::*[http://partsregistry.org/Part:BBa_J04500| BBa_J04500](2006)
Line 133: Line 138:
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==reference==
 
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*L. Steindler.V.Venturi.:Detection of quorum sensing N-acyl homoserine lactone.FEMS Microbiol Lett '''266''' (2007) 1-9
 
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:クオラムセンシングのクロストークについてのミニレビュー。クオラムセンシングを利用したAHLバイオセンサーについて、Table 1.にまとまっている。
 
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*M.K Winson ''et al''.Construction and analysis of luxCDABE-based plasmid sensors　for investigating N-acyl homoserine lactone-mediated quorum sensing.FEMS Microbiology Letters 163 (1998) 185-192
 
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:Figure 2.hは、LuxR、RhlR、LasRそれぞれの、C4-C14のN-acyl-L-homoserine lactone,C4-C14のN-(3-oxoacyl)-L-homoserine lactoneに対する感受性を比較したグラフ。
 
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*Michiko E. Taga. Bonnie L. Bassler.:Chemical communication among bacteria.PNAS.November 25, 2003,'''100'''suppl.2
 
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:クオラムセンシングについての総説。アシル基の異なるAHL、およびそのシンターゼの紹介、AIPを用いたコミュニケーション、AiiA（AHL分解酵素）についてのまとめがある。
 
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*K.M.GRAY ''et al''.Interchangeability and Specificity of Components from the Quorum-Sensing Regulatory Systems of Vibrio fischeri and Pseudomonas aeruginosa.J.BACTERIOL.'''176''',1994,3076-3080
 
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:LasとLuxのクロストークについて調べた論文。Table 1とTable 2で、promotor、レセプタータンパク質、インデューサーそれぞれを交換して、レポーターの発現の様子を調べている。
 
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*FK Balagadde ''et al''.:A synthetic predator–prey ecosystem.Molecular Systems Biology 4:187,2008
 
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:LuxとLasによるクオラムセンシングを利用した、プレデター・プレイモデル。LuxとLasのクロストークについて言及あり。
 
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*Rong-guang Zhang ''et al''.:Structure of a bacterial quorumsensing transcription factor complexed with pheromone and DNA.NATURE '''417''',2002,971-974
 
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:LuxR-familyタンパク質のTraR(Agrobacterium tumefaciens)の構造について、詳細な記述があります。
 
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{| style="color:white;background-color:Maroon" cellpadding="3" cellspacing="3" border="1" bordercolor="white" width="100%" align="center"

Revision as of 14:03, 30 August 2008

β班の実験ノート


Contents

Week 1

  • 17 August, 2008
Transformation
  • [http://partsregistry.org/Part:BBa_R0040| BBa_R0040](2008) -> No colonies on plates
  • [http://partsregistry.org/Part:BBa_C0070| BBa_C0070](2008) -> No colonies on plates
  • [http://partsregistry.org/Part:BBa_C0076| BBa_C0076](2008) -> No colonies on plates
  • [http://partsregistry.org/Part:BBa_C0077| BBa_C0077](2008) -> No colonies on plates
  • [http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2008) -> No colonies on plates
  • [http://partsregistry.org/Part:BBa_C0079| BBa_C0079](2008) -> No colonies on plates


  • 18 August, 2008
Transformation
  • [http://partsregistry.org/Part:BBa_C0070| BBa_C0070](2007)
  • [http://partsregistry.org/Part:BBa_C0070| BBa_C0070](2006)
  • [http://partsregistry.org/Part:BBa_C0076| BBa_C0076](2007)
  • [http://partsregistry.org/Part:BBa_C0076| BBa_C0076](2006) -> No colonies on plates
  • [http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2007)
  • [http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2006)
  • [http://partsregistry.org/Part:BBa_C0170| BBa_C0170](2007)
  • [http://partsregistry.org/Part:BBa_C0170| BBa_C0170](2006)
  • [http://partsregistry.org/Part:BBa_C0178| BBa_C0178](2007)
  • [http://partsregistry.org/Part:BBa_C0178| BBa_C0178](2006)


  • 19 August, 2008
PCR
  • [http://partsregistry.org/Part:BBa_I9026| BBa_I9026](2007)
  • [http://partsregistry.org/Part:BBa_I9026| BBa_I9026](2006)
  • [http://partsregistry.org/Part:BBa_I9030| BBa_I9030](2007)
  • [http://partsregistry.org/Part:BBa_I9030| BBa_I9030](2006)
  • [http://partsregistry.org/Part:BBa_S3154| BBa_S3154](2007)
  • [http://partsregistry.org/Part:BBa_S3154| BBa_S3154](2006)
  • [http://partsregistry.org/Part:BBa_F2620| BBa_F2620](2007)･･･control
Gel Check
  • [http://partsregistry.org/Part:BBa_I9026| BBa_I9026](2007)
  • [http://partsregistry.org/Part:BBa_I9026| BBa_I9026](2006)
  • [http://partsregistry.org/Part:BBa_I9030| BBa_I9030](2007)
  • [http://partsregistry.org/Part:BBa_I9030| BBa_I9030](2006)
  • [http://partsregistry.org/Part:BBa_S3154| BBa_S3154](2007)
  • [http://partsregistry.org/Part:BBa_S3154| BBa_S3154](2006)
  • [http://partsregistry.org/Part:BBa_F2620| BBa_F2620](2007)


  • 20 August, 2008
Mini prep
  • [http://partsregistry.org/Part:BBa_C0070| BBa_C0070](2007)
  • [http://partsregistry.org/Part:BBa_C0070| BBa_C0070](2006)
  • [http://partsregistry.org/Part:BBa_C0076| BBa_C0076](2007)
  • [http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2007)
  • [http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2006)
Digestion
  • [http://partsregistry.org/Part:BBa_C0170| BBa_C0170](2007)
  • [http://partsregistry.org/Part:BBa_C0170| BBa_C0170](2006)
  • [http://partsregistry.org/Part:BBa_C0178| BBa_C0178](2007)
  • [http://partsregistry.org/Part:BBa_C0178| BBa_C0178](2006)
Gel Check
  • [http://partsregistry.org/Part:BBa_C0070| BBa_C0070](2007)
  • [http://partsregistry.org/Part:BBa_C0070| BBa_C0070](2006)
  • [http://partsregistry.org/Part:BBa_C0076| BBa_C0076](2007)
  • [http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2007)
  • [http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2006)


  • 21 August, 2008
PCR
  • [http://partsregistry.org/Part:BBa_C0070| BBa_C0070](2007)
  • [http://partsregistry.org/Part:BBa_C0070| BBa_C0070](2006)
  • [http://partsregistry.org/Part:BBa_C0076| BBa_C0076](2007)
  • [http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2007)
  • [http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2006)
Gel Check
  • [http://partsregistry.org/Part:BBa_C0070| BBa_C0070](2007)
  • [http://partsregistry.org/Part:BBa_C0070| BBa_C0070](2006)
  • [http://partsregistry.org/Part:BBa_C0076| BBa_C0076](2007) -> None
  • [http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2007) -> None
  • [http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2006) -> None
Transformation
  • [http://partsregistry.org/Part:BBa_S03154| BBa_S03154](2007)
  • [http://partsregistry.org/Part:BBa_S03154| BBa_S03154](2006)
  • [http://partsregistry.org/Part:BBa_I9026| BBa_I9026](2007)
  • [http://partsregistry.org/Part:BBa_I9026| BBa_I9026](2006)
  • [http://partsregistry.org/Part:BBa_I9030| BBa_I9030](2007)
  • [http://partsregistry.org/Part:BBa_I9030| BBa_I9030](2006)
  • 22 August, 2008
Transformation
  • [http://partsregistry.org/Part:BBa_J13002| BBa_J13002](2007)
  • [http://partsregistry.org/Part:BBa_J13002| BBa_J13002](2006)
  • [http://partsregistry.org/Part:BBa_J04500| BBa_J04500](2007)
  • [http://partsregistry.org/Part:BBa_J04500| BBa_J04500](2006)
(Locate & 2 Punch ~iyama ver.~)
  • [http://partsregistry.org/Part:BBa_C0076| BBa_C0076](2008) -> No colonies on plates
  • [http://partsregistry.org/Part:BBa_C0077| BBa_C0077](2008) -> No colonies on plates
  • [http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2008) -> No colonies on plates
  • 23 August, 2008

Week 2

  • 24 August, 2008
Mini prep
  • [http://partsregistry.org/Part:BBa_J13002| BBa_J13002](2007)
  • [http://partsregistry.org/Part:BBa_J13002| BBa_J13002](2006)
  • [http://partsregistry.org/Part:BBa_J04500| BBa_J04500](2007)
  • [http://partsregistry.org/Part:BBa_J04500| BBa_J04500](2006)


  • 25 August, 2008


  • 26 August, 2008


  • 27 August, 2008
PCR
Colony PCR of 5 colonies from ligation plates (27/8) and one from control plate([http://partsregistry.org/Part:BBa_F2620| BBa_F2620](2007)).


  • 30 August, 2008


Week 3

  • 31 August, 2008


  • 1 September, 2008

Week 4

ホーム メンバー紹介 プロジェクト紹介 Parts Submitted to the Registry モデリング ノート
Retrieved from "http://2008.igem.org/Team:Chiba/jk/%CE%B2"