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| - | '''--[[User:Aronlau|Aronlau]] 00:35, 5 June 2008 (UTC)'''
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| - | <pre>
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| - | PCR al0001/al0002 on pSB3C6-Bjh1501 (508bp, EcoRI/BamHI/DpnI)
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| - | Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
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| - | Product is pBca1256-K112300
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| - | -----------------------------------------------
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| - | al0001 Forward Biobricking of lambda lysozyme with start and stop codons
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| - | CCATAGAATTCatgAGATCTatggtagaaatcaataatcaacgtaaggcgttcctcg
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| - | al0002 Reverse Biobricking of lambda lysozyme with start and stop codons
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| - | CGttaGGATCCtcatacatcaatctctctgaccgttccgcc
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| - | </pre>
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| | | | |
| - | <pre>
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| - | PCR al0003/al0002 on pSB3C6-Bjh1501 (505bp, EcoRI/BamHI/DpnI)
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| - | Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
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| - | Product is pBca1256-K112301
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| - | -----------------------------------------------
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| - | al0003 Forward Biobricking of Lambda Lysozyme with stop codon and no start codon
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| - | CCATAGAATTCatgAGATCTgtagaaatcaataatcaacgtaaggcgttcctcg
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| - | al0002 Reverse Biobricking of Lambda Lysozyme with stop codon and no start codon
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| - | CGttaGGATCCtcatacatcaatctctctgaccgttccgcc
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| - | </pre>
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| - |
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| - | <pre>
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| - | PCR al0001/al0004 on pSB3C6-Bjh1501 (505bp, EcoRI/BamHI/DpnI)
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| - | Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
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| - | Product is pBca1256-K112302
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| - | -----------------------------------------------
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| - | al0001 Forward Biobricking of lambda lysozyme with start codon and no start codon
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| - | CCATAGAATTCatgAGATCTatggtagaaatcaataatcaacgtaaggcgttcctcg
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| - | al0004 Reverse Biobricking of lambda lysozyme with start codon and no start codon
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| - | CGttaGGATCCtacatcaatctctctgaccgttccgcc
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| - | </pre>
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| - |
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| - | <pre>
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| - | PCR al0003/al0004 on pSB3C6-Bjh1501 (502bp, EcoRI/BamHI/DpnI)
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| - | Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
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| - | Product is pBca1256-K112303
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| - | -----------------------------------------------
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| - | al0003 Forward Biobricking of lambda lysozyme with no start/stop codon
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| - | CCATAGAATTCatgAGATCTgtagaaatcaataatcaacgtaaggcgttcctcg
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| - | al0004 Reverse Biobricking of lambda lysozyme with no start/stop codon
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| - | CGttaGGATCCtacatcaatctctctgaccgttccgc
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| - | </pre>
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| - |
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| - | <pre>
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| - | PCR al0005/al0002 on pSB3C6-Bjh1501 (512bp, EcoRI/BamHI/DpnI)
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| - | Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
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| - | Product is pBca1256-K112304
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| - | -----------------------------------------------
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| - | al0005 Forward Biobricking of lambda lysozyme with spacer
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| - | CCATAGAATTCatgAGATCTacatatggtagaaatcaataatcaacgtaaggcgttcctcg
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| - | al0002 Reverse Biobricking of lambda lysozyme with spacer
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| - | CGttaGGATCCtcatacatcaatctctctgaccgttccgcc
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| - | </pre>
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| - |
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| - | <pre>
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| - | PCR al0006/al0002 on pSB3C6-Bjh1501 (527bp, EcoRI/BamHI/DpnI)
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| - | Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
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| - | Product is pBca1256-K112305
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| - | -----------------------------------------------
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| - | al0006 Forward Biobricking of lambda lysozyme with rbs site
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| - | CCATAGAATTCatgAGATCTaaaaaagccggagtagaagatgg
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| - | al0002 Reverse Biobricking of lambda lysozyme with rbs site
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| - | CGttaGGATCCtcatacatcaatctctctgaccgttccgcc
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| - | </pre>
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| - |
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| - | <pre>
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| - | PCR al0007/al0008 on pSB3C6-Bjh1502 (349bp, EcoRI/BamHI/DpnI)
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| - | Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
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| - | Product is pBca1256-K112306
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| - | -----------------------------------------------
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| - | al0007 Forward Biobricking of lambda holin with start and stop codons
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| - | CCATAGAATTCatgAGATCTatgccagaaaaacatgacctg
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| - | al0008 Reverse Biobricking of lambda holin with start and stop codons
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| - | CGttaGGATCCttattgatttctaccatcttctactccggc
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| - | </pre>
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| - |
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| - | <pre>
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| - | PCR al0009/al0008 on pSB3C6-Bjh1502 (346bp, EcoRI/BamHI/DpnI)
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| - | Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
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| - | Product is pBca1256-K112307
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| - | -----------------------------------------------
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| - | al0009 Forward Biobricking of lambda holin with stop codon and no start codon
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| - | CCATAGAATTCatgAGATCTccagaaaaacatgacctg
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| - | al0008 Reverse Biobricking of lambda holin with stop codon and no start codon
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| - | CGttaGGATCCttattgatttctaccatcttctactccggc
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| - | </pre>
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| - |
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| - | <pre>
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| - | PCR al0007/al0010 on pSB3C6-Bjh1502 (346bp, EcoRI/BamHI/DpnI)
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| - | Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
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| - | Product is pBca1256-K112308
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| - | -----------------------------------------------
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| - | al0007 Forward Biobricking of lambda holin with start and no stop codon
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| - | CCATAGAATTCatgAGATCTatgccagaaaaacatgacctg
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| - | al0010 Reverse Biobricking of lambda holin wtih start and no stop codon
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| - | CGttaGGATCCttgatttctaccatcttctactccggc
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| - | </pre>
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| - |
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| - | <pre>
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| - | PCR al0009/al0010 on pSB3C6-Bjh1502 (343bp, EcoRI/BamHI/DpnI)
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| - | Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
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| - | Product is pBca1256-K112309
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| - | -----------------------------------------------
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| - | al0009 Forward Biobricking of lambda holin with no start and stop codon
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| - | CCATAGAATTCatgAGATCTccagaaaaacatgacctg
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| - | al0010 Reverse Biobricking of lambda holin with no start and stop codon
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| - | CGttaGGATCCttgatttctaccatcttctactccggc
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| - | </pre>
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| - |
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| - | <pre>
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| - | PCR al0011/al0008 on pSB3C6-Bjh1502 (353bp, EcoRI/BamHI/DpnI)
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| - | Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
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| - | Product is pBca1256-K112310
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| - | -----------------------------------------------
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| - | al0011 Forward Biobricking of lambda holin with spacer
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| - | CCATAGAATTCatgAGATCTacatatgccagaaaaacatgacctg
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| - | al0008 Reverse Biobricking of lambda holin with spacer
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| - | CGttaGGATCCttattgatttctaccatcttctactccggc
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| - | </pre>
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| - |
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| - | <pre>
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| - | PCR al0012/al0008 on pSB3C6-Bjh1502 (369bp, EcoRI/BamHI/DpnI)
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| - | Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
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| - | Product is pBca1256-K112311
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| - | -----------------------------------------------
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| - | al0012 Forward Biobricking of lambda holin with rbs site
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| - | ???
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| - | al0008 Reverse Biobricking of lambda holin with rbs site
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| - | CGttaGGATCCttattgatttctaccatcttctactccggc
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| - | </pre>
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| - |
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| - | <pre>
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| - | PCR al0013/al0014 on pSB3C6-Bjh1503 (355bp, EcoRI/BamHI/DpnI)
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| - | Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
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| - | Product is pBca1256-K112312
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| - | -----------------------------------------------
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| - | al0013 Forward Biobricking of lambda antiholin with start and stop codons
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| - | CCATAGAATTCatgAGATCTatgaagCtgccagaaaaacatgacc
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| - | al0014 Reverse Biobricking of lambda antiholin with start and stop codons
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| - | CGttaGGATCCttattgatttctaccatcttctactccggc
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| - | </pre>
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| - |
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| - | <pre>
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| - | PCR al0015/al0014 on pSB3C6-Bjh1503 (352bp, EcoRI/BamHI/DpnI)
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| - | Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
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| - | Product is pBca1256-K112313
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| - | -----------------------------------------------
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| - | al0015 Forward Biobricking of lambda antiholin with stop codon and no start codon
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| - | CCATAGAATTCatgAGATCTaagCtgccagaaaaacatgacctg
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| - | al0014 Reverse Biobricking of lambda antiholin with stop codon and no start codon
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| - | CGttaGGATCCttattgatttctaccatcttctactccggc
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| - | </pre>
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| - |
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| - | <pre>
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| - | PCR al0013/al0016 on pSB3C6-Bjh1503 (352bp, EcoRI/BamHI/DpnI)
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| - | Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
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| - | Product is pBca1256-K112314
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| - | -----------------------------------------------
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| - | al0013 Forward Biobricking of lambda antiholin with start codon and no stop codon
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| - | CCATAGAATTCatgAGATCTatgaagCtgccagaaaaacatgacc
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| - | al0016 Reverse Biobricking of lambda antiholin with start codon and no stop codon
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| - | CGttaGGATCCttgatttctaccatcttctactccgg
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| - | </pre>
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| - |
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| - | <pre>
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| - | PCR al0015/al0016 on pSB3C6-Bjh1503 (349bp, EcoRI/BamHI/DpnI)
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| - | Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
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| - | Product is pBca1256-K112315
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| - | -----------------------------------------------
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| - | al0015 Forward Biobricking of lambda antiholin with no start and stop codons
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| - | CCATAGAATTCatgAGATCTaagCtgccagaaaaacatgacctg
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| - | al0016 Reverse Biobricking of lambda antiholin with no start and stop codons
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| - | ???
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| - | </pre>
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| - |
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| - | <pre>
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| - | PCR al0017/al0014 on pSB3C6-Bjh1503 (359bp, EcoRI/BamHI/DpnI)
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| - | Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
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| - | Product is pBca1256-K112316
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| - | -----------------------------------------------
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| - | al0017 Forward Biobricking of lambda antiholin with spacer
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| - | al0014 Reverse Biobricking of lambda antiholin with spacer
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| - | CGttaGGATCCttattgatttctaccatcttctactccggc
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| - | </pre>
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| - |
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| - | <pre>
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| - | PCR al0018/al0014 on pSB3C6-Bjh1503 (375bp, EcoRI/BamHI/DpnI)
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| - | Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
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| - | Product is pBca1256-K112317
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| - | -----------------------------------------------
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| - | al0018 Forward Biobricking of lambda antiholin with rbs site
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| - | CCATAGAATTCatgAGATCTccccttattgggggtaagacatgaagCtgccagaaaaacatgacc
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| - | al0014 Reverse Biobricking of lambda antiholin with rbs site
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| - | CGttaGGATCCttattgatttctaccatcttctactccggc
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| - | </pre>
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"
