Purdue/7 August 2008
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+ | Jess digested o/n the mCherry parts to see if they're there... | ||
+ | |||
+ | *10uL DNA | ||
+ | *2uL Buffer #2 | ||
+ | *8uL Mix (recipe below for 2 tubes) | ||
+ | **13uL ddH2O | ||
+ | **1uL EcoRI | ||
+ | **1uL SpeI | ||
+ | **1uL BSA | ||
+ | |||
+ | *Put at 37C o/n | ||
+ | *Ran on gel w/ Fast Blast | ||
+ | *Found out that Fast Blast makes DNA Positive! (didn't work...) | ||
+ | |||
+ | |||
+ | '''Edited by Janie Stine''' |
Latest revision as of 15:30, 2 September 2008
Click Here to return to the notebook.
Jess digested o/n the mCherry parts to see if they're there...
- 10uL DNA
- 2uL Buffer #2
- 8uL Mix (recipe below for 2 tubes)
- 13uL ddH2O
- 1uL EcoRI
- 1uL SpeI
- 1uL BSA
- Put at 37C o/n
- Ran on gel w/ Fast Blast
- Found out that Fast Blast makes DNA Positive! (didn't work...)
Edited by Janie Stine