Team:Hawaii/Notebook/2008-09- 3

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(New page: {{Team:Hawaii/Header}} = Things we did today = == Wetlab work == ===Re-replacement of BB-pRL1383a MCS=== :<strong> Grace</strong> [[Image:090308REdigest.jpg|right|thumb|200px|EtBr stained...)
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Revision as of 18:03, 3 September 2008

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Things we did today

Wetlab work

Re-replacement of BB-pRL1383a MCS

Grace
EtBr stained 0.8% agarose gel ran at 60V for ---. Thirty microliters of RE digest reaction were loaded into each well.
  • Ran last night's RE digest on a gel
  • Extracted bands from gel
  • Treated BB-pRL1383a w/ SAP
  • Ligated --μl J33207 w/ --μl BB-pRL1383a
  • Transformed DH5α cells using 5 μl ligation reaction


Discussion

Quote of the Day

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson


[http://manoa.hawaii.edu/ Sponsor_UHM.gif][http://manoa.hawaii.edu/ovcrge/ Sponsor_OVCRGE.gif][http://www.ctahr.hawaii.edu Sponsor_CTAHR.gif]


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