Revision as of 01:22, 4 September 2008

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Things we did today

Grace

EtBr stained 0.8% agarose gel ran at 60V for 1.5 hours. Thirty microliters of RE digested BB-pRL1383a were loaded onto the gel.

Grace

C0012V (SAP treated) + plac + rbs (B0034 back ligation)
C0012V + plac + rbs (B0034 back ligation)
Negative controls = C0012V (SAP treated and no SAP treatment) ligated to self, plac + rbs ligated w/o vector

name of person/people who performed the task

Summary of task and what was done. Link to experiment for detailed notes if necessary.
e.g. read through papers, worked on proposal, etc.

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson

[http://manoa.hawaii.edu/ ][http://manoa.hawaii.edu/ovcrge/ ][http://www.ctahr.hawaii.edu ]

@@ Line 5: / Line 5: @@
 ===Re-replacement of BB-pRL1383a MCS===
 :<strong> Grace</strong>
+[[Image:090408BBpRL.jpg|right|thumb|200px|EtBr stained 0.8% agarose gel ran at 60V for 1.5 hours. Thirty microliters of RE digested BB-pRL1383a were loaded onto the gel.]]
-:* Treated inactivated RE digest reaction with SAP
+:* Ran last night's RE digest on agarose gel
+:* Extracted J33207 and BB-pRL1383a from gel
+:* Treated BB-pRL1383a w/ SAP
 :* Checked DNA concentration of BB-pRL1383a (---ng/&mu;l)
 :* Ligated J33207 with BB-pRL1383a