Team:Paris/Modeling/f3bis

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(Difference between revisions)
Line 36: Line 36:
|γ<sub>GFP</sub>
|γ<sub>GFP</sub>
|dilution-degradation rate <br> of GFP(mut3b)
|dilution-degradation rate <br> of GFP(mut3b)
-
|s<sup>-1</sup>
+
|min<sup>-1</sup>
-
|ln(2)/3600
+
|0.0198
|-
|-
|[GFP]
|[GFP]
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|β<sub>12bis</sub>
|β<sub>12bis</sub>
|production rate of FliA-pFlhDC '''with RBS E0032''' <br> replace β<sub>12</sub> (not written)
|production rate of FliA-pFlhDC '''with RBS E0032''' <br> replace β<sub>12</sub> (not written)
-
|nM.s<sup>-1</sup>
+
|nM.min<sup>-1</sup>
|
|
|-
|-
-
|(K<sub>11bis</sub>/{coef<sub>fliA</sub>}<sup>n<sub>11bis</sub></sup>)
+
|(K<sub>11bis</sub>/{coef<sub>fliA</sub>})
|activation constant of FliA-pFlhDC <br> K<sub>11bis</sub>
|activation constant of FliA-pFlhDC <br> K<sub>11bis</sub>
-
|nM<sup>n<sub>11bis</sub></sup>
+
|nM
|
|
|-
|-
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|β<sub>2bis</sub>
|β<sub>2bis</sub>
|production rate of EnvZ-pFlhDC '''with RBS E0032''' <br> β<sub>2bis</sub>
|production rate of EnvZ-pFlhDC '''with RBS E0032''' <br> β<sub>2bis</sub>
-
|nM.s<sup>-1</sup>
+
|nM.min<sup>-1</sup>
|
|
|-
|-
-
|(K<sub>19bis</sub>/{coef<sub>envZ</sub>}<sup>n<sub>19bis</sub></sup>)
+
|(K<sub>19bis</sub>/{coef<sub>envZ</sub>})
|activation constant of EnvZ-pFlhDC <br> replace K<sub>19</sub> (not written)
|activation constant of EnvZ-pFlhDC <br> replace K<sub>19</sub> (not written)
-
|nM<sup>n<sub>19bis</sub></sup>
+
|nM
|
|
|-
|-

Revision as of 06:35, 5 September 2008

F3env.png
At the steady-state, we have
FliAeq.jpg

and

EnvZeq.jpg

so the expression

F3envexpr.jpg

gives

F3bisFliA.jpg

and

F3bisEnvZ.jpg

and for calculated values of the TF,

F3bisFliACalc.jpg

and

F3bisEnvZCalc.jpg



param signification unit value
[expr(pFlhDC)] expression rate of
pFlhDC with RBS E0032
nM.s-1 see "findparam"
need for 20 + 20 measures
and 5x5 measures for the SUM?
γGFP dilution-degradation rate
of GFP(mut3b)
min-1 0.0198
[GFP] GFP concentration at steady-state nM need for 20 + 20 measures
and 5x5 measures for the SUM?
(fluorescence) value of the observed fluorescence au need for 20 + 20 measures
and 5x5 measures for the SUM?
conversion conversion ration between
fluorescence and concentration
nM.au-1 (1/79.429)



param signification
corresponding parameters in the equations
unit value
β12bis production rate of FliA-pFlhDC with RBS E0032
replace β12 (not written)
nM.min-1
(K11bis/{coeffliA}) activation constant of FliA-pFlhDC
K11bis
nM
n11bis complexation order of FliA-pFlhDC
replace n11 (not written)
no dimension
β2bis production rate of EnvZ-pFlhDC with RBS E0032
β2bis
nM.min-1
(K19bis/{coefenvZ}) activation constant of EnvZ-pFlhDC
replace K19 (not written)
nM
n19bis complexation order of EnvZ-pFlhDC
n19bis
no dimension



Then, if we have time, we want to verify the expected relation

SumpFlhDC2.jpg