Team:University of Ottawa/4 June 2008

From 2008.igem.org

(Difference between revisions)
(Today in the lab)
(Today in the lab)
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==Today in the lab==
==Today in the lab==
'''Dan, Matt'''
'''Dan, Matt'''
-
:<li>Miniprep was performed for all four plasmids and concentrations of DNA were calculated.
+
:'''Miniprep'''
-
:<li>Digestion volumes were calculated, DNA was then left to digest overnight
+
::<li>Miniprep was performed for all four plasmids and concentrations of DNA were calculated - 1B was very low so it might not turn out on the gel.
 +
:'''Digestion'''
 +
::<li>Digestion volumes were calculated, DNA was then left to digest overnight in the PCR machine.

Revision as of 18:31, 6 June 2008

Today in the lab

Dan, Matt

Miniprep
  • Miniprep was performed for all four plasmids and concentrations of DNA were calculated - 1B was very low so it might not turn out on the gel.
  • Digestion
  • Digestion volumes were calculated, DNA was then left to digest overnight in the PCR machine.