Team:Hawaii/Notebook/2008-09-20

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Revision as of 23:01, 20 September 2008

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Things we did today

Wetlab work

Construction of secretion device (cont.)

Grace
EtBr stained 4% agarose gels ran at 60V for 2.5 hours. Twenty five microliters of RE digest were loaded into each well.
  • Ran RE digests on 4% agarose gel
  • Extracted bands from gel

Drylab Work

Sequencing

Grace & Krystle
  • Figured out Krystle's sequencing results
  • GFPf+tt 17F+8R = ccdB base vector
  • GFPf+tt 8F+21R = rbs (B0030+GFPf)
  • GFPF+tt 21F+17R = GFPf+B1006
  • Need to resequence #21, 17 because no way to tell which is correct (F/R)

Discussion

Quote of the Day

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson


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