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Team:Hawaii/Notebook/2008-09-19
From 2008.igem.org
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(→Drylab Work) |
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::* EcoRI/PstI | ::* EcoRI/PstI | ||
:::* pSB1A3 | :::* pSB1A3 | ||
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| + | :<strong> Krystle </strong> | ||
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| + | :*Transformation of overnight ligations | ||
===Gel to confirm transformants=== | ===Gel to confirm transformants=== | ||
Latest revision as of 01:37, 21 September 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Plasmid prep of BB-pRL1383a
- Grace
Construction of secretion device
- Grace
- PCR of nir+rbs, rbs+GFP, slr1+GFPf, pilA, slr2
- Overnight RE digest:
- EcoRI/SpeI
- nir+rbs
- rbs+GFP
- slr1+GFPf
- nir (PCR product from before)
- XbaI/PstI
- rbs+GFP
- slr1+GFPf
- pilA
- slr2
- EcoRI/PstI
- pSB1A3
- Krystle
- Transformation of overnight ligations
Gel to confirm transformants
Margaret
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
