Team:UNIPV-Pavia/Protocols
From 2008.igem.org
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<h1>The protocols we used</h1> | <h1>The protocols we used</h1> | ||
This page will be updated soon with some links. | This page will be updated soon with some links. | ||
- | *Plasmid resuspention from IGEM paper spots | + | *[[Team:UNIPV-Pavia/Protocols/plasmid_resusp|Plasmid resuspention from IGEM paper spots]] |
*Transformation | *Transformation | ||
*Plasmid extraction | *Plasmid extraction | ||
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<br> | <br> | ||
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+ | {|align="center" cellpadding="30" | ||
+ | !|[[Team:UNIPV-Pavia/Notebook/Week1|Week 1]] | ||
+ | !|[[Team:UNIPV-Pavia/Notebook/Week2|Week 2]] | ||
+ | !|[[Team:UNIPV-Pavia/Notebook/Week3|Week 3]] | ||
+ | !|[[Team:UNIPV-Pavia/Notebook/Week4|Week 4]] | ||
+ | !|[[Team:UNIPV-Pavia/Notebook/Week5|Week 5]] | ||
+ | !|[[Team:UNIPV-Pavia/Notebook/Week6|Week 6]] | ||
+ | !|[[Team:UNIPV-Pavia/Notebook/Week7|Week 7]] | ||
+ | |} | ||
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+ | <br><br> | ||
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+ | ==Week 3: 06/03/08 - 06/08/08== | ||
+ | |||
+ | '''06/03/08''' | ||
+ | <br> | ||
+ | *We transformed 60 µl of TOP10 with 2 µl of the 6 parts (DNA + glycerol) we received from IGEM HQ: | ||
+ | |||
+ | {|cellpadding="20" | ||
+ | |BBa_C0179 | ||
+ | |BBa_C0161 |
Revision as of 10:46, 8 June 2008
Home | The Team | The Project | Biological Safety | Parts Submitted to the Registry |
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Dry Lab | Wet Lab | Modeling | Protocols | Activity Notebook |
The protocols we used
This page will be updated soon with some links.
- Plasmid resuspention from IGEM paper spots
- Transformation
- Plasmid extraction
- BioBrick digestion with restriction enzymes
- DNA gel extraction
- Antartic Phosphatase
- Ligation
Week 1 | Week 2 | Week 3 | Week 4 | Week 5 | Week 6 | Week 7 |
---|
Week 3: 06/03/08 - 06/08/08
06/03/08
- We transformed 60 µl of TOP10 with 2 µl of the 6 parts (DNA + glycerol) we received from IGEM HQ:
BBa_C0179 | BBa_C0161 |