"
Team:Hawaii/Notebook/2008-09-24
From 2008.igem.org
(Difference between revisions)
(New page: {{Team:Hawaii/Header}} = Things we did today = == Wetlab work == ===Verified plasmid preps=== :<strong> Grace</strong> :* PCR of plasmid preps from yesterday to verify inserts ::* Ran PC...) |
(→Construction of secretion device (cont.)) |
||
| Line 17: | Line 17: | ||
:* Overnight RE digest: | :* Overnight RE digest: | ||
| - | ::* nrsg and nrg PCR products with EcoRI and SpeI | + | ::* nir, nrsg, and nrg PCR products with EcoRI and SpeI |
::* rgt PCR products with XbaI and PstI | ::* rgt PCR products with XbaI and PstI | ||
::* J33207 PCR product and pRL1383a plasmid with HindIII and BamHI | ::* J33207 PCR product and pRL1383a plasmid with HindIII and BamHI | ||
Revision as of 00:24, 25 September 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Verified plasmid preps
- Grace
- PCR of plasmid preps from yesterday to verify inserts
- Ran PCR products on gel
- nrg #1 and nrsg #2 returned blanks. Bad plasmid prep?
- BB-pRL1383a and colony PCR of BB-pRL1383a E. coli both blank. Yikes!
- Quantified BB-pRL1383a and pSB1A3 plasmid preps
- Blanks. Really low conc.? Will nanodrop to check.
Construction of secretion device (cont.)
- Grace
- Overnight RE digest:
- nir, nrsg, and nrg PCR products with EcoRI and SpeI
- rgt PCR products with XbaI and PstI
- J33207 PCR product and pRL1383a plasmid with HindIII and BamHI
Prep for sequencing
- Grace
- nrg #4, nrg #7, nrsg #8, rgt #1, rgt #2
- plac+rbs
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
