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Team:Purdue/Modeling
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<!--- The Mission, Experiments ---> | <!--- The Mission, Experiments ---> | ||
Revision as of 16:13, 25 September 2008
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Modeling Objectives
- Develop a mechanistics ODE model of the population to predict gene expression dynamics
- Question? .... Will the color production be fast enough to be useful to the user? Or will it be too late?
- What is the relationship between UV exposure and reporter gene expression?
- Can we construct a useful calibration curve of color as a function of UV?
Modeling References
- 1997. "Mathematical model of the SOS response regulation of an excision repair deficient mutant of Escherichia coli after UV light irradation". [http://www2.lib.purdue.edu:2184/science?_ob=ArticleURL&_udi=B6WMD-45KKS62-5S&_user=29441&_coverDate=05%2F21%2F1997&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_version=1&_urlVersion=0&_userid=29441&md5=7297869ffc31d1edfcd20856301793a5]. Off-the-shelf mechanistic model of the SOS response. Utilized as the basic model for our system.
- 2005. "Response times and mechanisms of SOS induction by attaching promoters to GFP: "Precise Temporal Modulation in the Response of the SOS DNA Repair Network in Individual Bacteria" [http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1151601]. Potential Validation Data Set. Model should predict similar dynamics.
Modeling the SOS response in a uvr- mutant (No nucleotide excision repair)
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