Team:Warsaw/Calendar-Main/11 June 2008

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(Difference between revisions)
(New page: {{WarNotebook}} <!-- do not edit above me! --> <p>pMPMT5, pMPMT5-AID, pMPMT5-AIDT7 and pMPMT5-AiD+T7 were transformed to E.coli Top10 carrying pZC. transformants were plated on 'TAXI' s...)
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<p>pMPMT5, pMPMT5-AID, pMPMT5-AIDT7 and pMPMT5-AiD+T7 were transformed to E.coli Top10 carrying pZC. transformants were plated on 'TAXI' solid medium (LB + Tetracycline + IPTG + Ampicillin 30 μg/mL + X-Gal) </p>
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<p>Transformation of E.coli Top10 carrying pZC320 with pMPMT5, pMPMT5-AID, pMPMT5-AIDT7 and pMPMT5-AiD+T7.
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Plating transformants on 'TAXI'(LB + Tetracycline + IPTG + Ampicillin 30 μg/mL + X-Gal).</p>

Revision as of 13:56, 26 September 2008

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Transformation of E.coli Top10 carrying pZC320 with pMPMT5, pMPMT5-AID, pMPMT5-AIDT7 and pMPMT5-AiD+T7. Plating transformants on 'TAXI'(LB + Tetracycline + IPTG + Ampicillin 30 μg/mL + X-Gal).

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