Team:Warsaw/Calendar-Main/11 June 2008
From 2008.igem.org
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- | <p>Transformation of E.coli Top10 carrying pZC320 with pMPMT5, pMPMT5-AID, pMPMT5-AIDT7 | + | <p>Transformation of E.coli Top10 carrying pZC320 with pMPMT5, pMPMT5-AID, pMPMT5-AIDT7 or pMPMT5-AiD+T7. |
- | Plating transformants on 'TAXI'(LB + Tetracycline + IPTG + Ampicillin 30 μg/mL + X-Gal).</p> | + | Plating transformants on 'TAXI' (LB + Tetracycline + IPTG + Ampicillin 30 μg/mL + X-Gal).</p> |
Revision as of 13:57, 26 September 2008
Transformation of E.coli Top10 carrying pZC320 with pMPMT5, pMPMT5-AID, pMPMT5-AIDT7 or pMPMT5-AiD+T7. Plating transformants on 'TAXI' (LB + Tetracycline + IPTG + Ampicillin 30 μg/mL + X-Gal).
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