Judging/Variance/Brown

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(New page: Dear iGEM Judging Corps, Here at Brown, we are building a gene network in yeast. We are constructing our parts using the Silver Lab biofusion standard, but in many cases have been d...)
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Revision as of 15:34, 30 September 2008

Dear iGEM Judging Corps,

  Here at Brown, we are building a gene network in yeast.  We are
constructing our parts using the Silver Lab biofusion standard, but
in many cases have been doing the final ligation step as a double
insert onto a yeast integration vector from the pRS family.  
(As detailed at the bottom of   
http://openwetware.org/wiki/Silver:_BB_Strategy)
  
  Thus, our basic parts and intermediates are on biobrick standard
vectors, but our final constructs are not.  The pRS vectors have
biobrick sites present, and lack the full prefix or suffix,
depending on the vector.  Our parts on pRS vectors can be used as-is
for yeast integration, switched onto biobrick vectors in one
ligation step, or in some cases used as inserts in biobrick
construction.  Would you like us to add these parts to the registry
though they are not suitable as vectors for biobrick assembly?

Thank you,
John Szymanski

Brown iGEM
Team Limiter