Judging/Variance/Brown

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Revision as of 22:37, 30 September 2008

Request

Dear Judges,

At Brown we are building a regulatory gene network in Saccharomyces cerevisiae, and are excited to contribute to the growing collection of yeast parts in the Registry. We are building our constructs using the Silver Lab Biofusion standard for the fusion of protein domains, and the Biobrick standard for the addition of regulatory regions. Construction takes place on Biobrick vectors, while the final constructs are cloned onto integrative yeast shuttle vectors from the pRS30x family. (http://www.genetics.org/cgi/reprint/122/1/19.pdf) The pRS vectors are not suitable for Biobrick construction, but their multiple cloning sites provide for the convenient cloning of a complete Biobrick construct onto the vector. (http://openwetware.org/wiki/Silver:_BB_Strategy) Our parts on pRS vectors are useful as complete constructs for integration into the yeast genome, and in that way would be a valuable addition to the registry. Some final constructs could be useful as construction intermediates as well- in these cases we would like to submit them on both pRS vectors for yeast integration as-is, and on standard Biobrick vectors for use in construction

Thank you, John Szymanski Brown iGEM Team Limiter

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 ===Request===
-Dear iGEM Judging Corps,
+Dear Judges,
-Here at Brown, we are building a gene network in yeast.  We are
+At Brown we are building a regulatory gene network in ''Saccharomyces cerevisiae'', and are excited to contribute to the growing collection of yeast parts in the Registry.  We are building our constructs using the Silver Lab Biofusion standard for the fusion of protein domains, and the Biobrick standard for the addition of regulatory regions.   Construction takes place on Biobrick vectors, while the final constructs are cloned onto integrative yeast shuttle vectors from the pRS30x family. (http://www.genetics.org/cgi/reprint/122/1/19.pdf)  The pRS vectors are not suitable for Biobrick construction, but their multiple cloning sites provide for the convenient cloning of a complete Biobrick construct onto the vector.  (http://openwetware.org/wiki/Silver:_BB_Strategy)  Our parts on pRS vectors are useful as complete constructs for integration into the yeast genome, and in that way would be a valuable addition to the registry.  Some final constructs could be useful as construction intermediates as well- in these cases we would like to submit them on both pRS vectors for yeast integration as-is, and on standard Biobrick vectors for use in construction
-constructing our parts using the Silver Lab biofusion standard, but
-in many cases have been doing the final ligation step as a double
-insert onto a yeast integration vector from the pRS family.  (As
-detailed at the bottom of of http://openwetware.org/wiki/Silver:_BB_Strategy)
-Thus, our basic parts and intermediates are on biobrick standard
-vectors, but our final constructs are not.  The pRS vectors have
-biobrick sites present, and lack the full prefix or suffix,
-depending on the vector.  Our parts on pRS vectors can be used as-is
-for yeast integration, switched onto biobrick vectors in one
-ligation step, or in some cases used as inserts in biobrick
-construction.  Would you like us to add these parts to the registry
-though they are not suitable as vectors for biobrick assembly?
 Thank you,
 John Szymanski
 Brown iGEM
 Team Limiter