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Team:Warsaw/Calendar-Main/1 July 2008
From 2008.igem.org
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<li> PCR on pBD (jaki numerek?) plasmid with | <li> PCR on pBD (jaki numerek?) plasmid with | ||
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<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmpaL_N">OmpaL_N</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmpaP_link">OmpaP_link</a> | <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmpaL_N">OmpaL_N</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmpaP_link">OmpaP_link</a> | ||
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primers (15 cycles, annealing duration 45 s, annealing temperature 63°C) </li> | primers (15 cycles, annealing duration 45 s, annealing temperature 63°C) </li> | ||
<li> PCR on pUC19 plasmid with | <li> PCR on pUC19 plasmid with | ||
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<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AlphaL_link">AlphaL_link</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AlphaP_XB">AlphaP_XB</a> | <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AlphaL_link">AlphaL_link</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AlphaP_XB">AlphaP_XB</a> | ||
| - | + | primers (20 cycles, annealing duration 45 s, annealing temperature 63°C)</li> | |
| - | primers (20 cycles, annealing | + | |
<li> PCR on pUC19 plasmid with | <li> PCR on pUC19 plasmid with | ||
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<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmegaL_link">OmegaL_link</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmegaP_EPB">OmegaP_EPB</a> | <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmegaL_link">OmegaL_link</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmegaP_EPB">OmegaP_EPB</a> | ||
| - | + | primers (20 cycles, annealing duration 30 s, annealing temperature 58°C)<br> | |
| - | primers (20 cycles, annealing duration 30 s, annealing temperature 58°C)< | + | As a result we got three PCR products: OmpA_linker and two fragments of TEM1 beta-lactamese: linker_alpha and linker_omega. </li> |
| - | As a result we got three PCR products: OmpA_linker and two fragments of TEM1 beta-lactamese: linker_alpha and linker_omega. < | + | |
<li> Gel electrphoresis of PCR products and <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">gel-out</a> of proper bands</li> | <li> Gel electrphoresis of PCR products and <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">gel-out</a> of proper bands</li> | ||
</ol></p> | </ol></p> | ||
Revision as of 16:12, 1 October 2008
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Preparation of constructs with OmpA protein fusions
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