Team:Warsaw/Calendar-Main/1 July 2008

From 2008.igem.org

(Difference between revisions)
Line 7: Line 7:
<p>
<p>
<ol>
<ol>
-
<li> PCR on pBD (jaki numerek?) plasmid with  
+
<li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> on pBD (jaki numerek?) plasmid with  
<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmpaL_N">OmpaL_N</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmpaP_link">OmpaP_link</a>
<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmpaL_N">OmpaL_N</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmpaP_link">OmpaP_link</a>
-
  primers (15 cycles, annealing duration 45 s, annealing temperature 63&deg;C) </li>
+
  primers (15 cycles, elongation duration 45 s, elongation temperature 63&deg;C) </li>
-
<li> PCR on pUC19 plasmid with  
+
<li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> on pUC19 plasmid with  
<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AlphaL_link">AlphaL_link</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AlphaP_XB">AlphaP_XB</a>
<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AlphaL_link">AlphaL_link</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AlphaP_XB">AlphaP_XB</a>
-
  primers (20 cycles, annealing duration 45 s, annealing temperature 63&deg;C)</li>
+
  primers (20 cycles, elongation duration 45 s, elongation temperature 63&deg;C)</li>
-
<li> PCR on pUC19 plasmid with  
+
<li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> on pUC19 plasmid with  
<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmegaL_link">OmegaL_link</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmegaP_EPB">OmegaP_EPB</a>
<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmegaL_link">OmegaL_link</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmegaP_EPB">OmegaP_EPB</a>
-
  primers (20 cycles, annealing duration 30 s, annealing temperature 58&deg;C)<br>
+
  primers (20 cycles, elongation duration 30 s, elongation temperature 58&deg;C)<br>
As a result we got three PCR products: OmpA_linker and two fragments of TEM1 beta-lactamese: linker_alpha and linker_omega. </li>
As a result we got three PCR products: OmpA_linker and two fragments of TEM1 beta-lactamese: linker_alpha and linker_omega. </li>
-
<li> Gel electrphoresis of PCR products and <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">gel-out</a> of proper bands</li>
+
<li> Gel electrophoresis of PCR products and <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">gel-out</a> of proper bands</li>
</ol></p>
</ol></p>
</html>
</html>

Revision as of 16:15, 1 October 2008

Gallery Bricks Notebook Team Project Home


Previous day
return to main notebook page
Previous entry
Interactive list of topics
next notebook entry

 



Preparation of constructs with OmpA protein fusions

  1. PCR on pBD (jaki numerek?) plasmid with OmpaL_N and OmpaP_link primers (15 cycles, elongation duration 45 s, elongation temperature 63°C)
  2. PCR on pUC19 plasmid with AlphaL_link and AlphaP_XB primers (20 cycles, elongation duration 45 s, elongation temperature 63°C)
  3. PCR on pUC19 plasmid with OmegaL_link and OmegaP_EPB primers (20 cycles, elongation duration 30 s, elongation temperature 58°C)
    As a result we got three PCR products: OmpA_linker and two fragments of TEM1 beta-lactamese: linker_alpha and linker_omega.
  4. Gel electrophoresis of PCR products and gel-out of proper bands

Retrieved from "http://2008.igem.org/Team:Warsaw/Calendar-Main/1_July_2008"