Team:Warsaw/Calendar-Main/11 June 2008

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<h3>Michał L., Ewa, Marcin</h3>
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<html><h3>Michał L., Ewa, Marcin</h3>
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<p>Transformation of E.coli Top10 carrying pZC320 with pMPMT5, pMPMT5-AID, pMPMT5-AIDT7 or pMPMT5-AiD+T7.
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Plating transformants on 'TAXI' (LB + Tetracycline + IPTG + Ampicillin 30 μg/mL + X-Gal).</p>
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Rifampicin test of transformants
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<p><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#chemotransform">Transformation</a> of E.coli Top10 carrying pZC320 with:
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<ul><li>pMPMT5,</li>
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<li>pMPMT5-AID,</li>
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<li>pMPMT5-AIDT7, </li>
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<li>or pMPMT5-AiD+T7.</li></ul>
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<p>Plating transformants on 'TAXI' (LB + Tetracycline + IPTG + Ampicillin 30 μg/mL + X-Gal).</p>
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<p><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#rif">Rifampicin test</a> of transformants.</p>
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Revision as of 18:32, 1 October 2008

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Michał L., Ewa, Marcin

Transformation of E.coli Top10 carrying pZC320 with:

  • pMPMT5,
  • pMPMT5-AID,
  • pMPMT5-AIDT7,
  • or pMPMT5-AiD+T7.

Plating transformants on 'TAXI' (LB + Tetracycline + IPTG + Ampicillin 30 μg/mL + X-Gal).

Rifampicin test of transformants.

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