"
Team:Warsaw/Calendar-Main/16 July 2008
From 2008.igem.org
(Difference between revisions)
PPrzanowski (Talk | contribs) |
|||
| Line 4: | Line 4: | ||
| - | <h3>Cloning of protein Z DNA to OmpA constructs</h3> | + | <h3>Cloning of protein Z DNA to OmpA constructs<br>Michał K.</h3> |
<p><ol> | <p><ol> | ||
<li> Isolation of plasmids from cultures inocluated on previous day (pACYC177+OmpA_Z_alpha). </li> | <li> Isolation of plasmids from cultures inocluated on previous day (pACYC177+OmpA_Z_alpha). </li> | ||
<li> Control digest of isolated plasmids with BamHI and SacI (we found 4 good clones).</li></ol></p> | <li> Control digest of isolated plasmids with BamHI and SacI (we found 4 good clones).</li></ol></p> | ||
| - | + | <br> | |
| - | + | <h3>Cloning of protein A DNA to geneart plasmid in place of protein Z DNA<br>Antoni</h3> | |
| - | + | <p><ol> | |
| - | + | <li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#electrotransform">Transformation</a> of <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top">TOP10</a> strain with ligation from previous day </li> | |
| + | </ol></p> | ||
<!-- do not remove this! --> | <!-- do not remove this! --> | ||
{{WarNotebookEnd}} | {{WarNotebookEnd}} | ||
Revision as of 21:36, 1 October 2008
 |
|
||||||||
 |
|
||||||||
Cloning of protein Z DNA to OmpA constructs
|
|
