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Team:Warsaw/Calendar-Main/1 August 2008
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<p>1. <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#chemotransform">Transformation</a> of E. coli TOP10 strain with ligations (pACYC177+OmpA_alpha + deltaA and pACYC177+OmpA_omega + deltaA). <br> | <p>1. <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#chemotransform">Transformation</a> of E. coli TOP10 strain with ligations (pACYC177+OmpA_alpha + deltaA and pACYC177+OmpA_omega + deltaA). <br> | ||
2. Transformants plating on LB + kanamycin. </p> | 2. Transformants plating on LB + kanamycin. </p> | ||
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| + | Oczyszczanie białek: A-alfa, Z-alfa i Z-omega | ||
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| + | Piotr | ||
| + | Zwirowanie bakterii z 1l, sonikacja ich (protokół) | ||
| + | zwirowanie sonikatu | ||
| + | w przypadku z-alfa i z-omega odżucenie supernatantu, zawieszenie osadu w wodzi i ponowne zwirowanie i odżucenie wody i zawieszenie w wodzie | ||
| + | w przypadku a-alfa zatrzymanie supernatantu odżucenie osadu | ||
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Revision as of 21:30, 2 October 2008
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1. Transformation of E. coli TOP10 strain with ligations (pACYC177+OmpA_alpha + deltaA and pACYC177+OmpA_omega + deltaA).
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