Team:Warsaw/Calendar-Main/1 August 2008

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<p>1. <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#chemotransform">Transformation</a> of E. coli TOP10 strain with ligations (pACYC177+OmpA_alpha + deltaA and pACYC177+OmpA_omega + deltaA). <br>
<p>1. <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#chemotransform">Transformation</a> of E. coli TOP10 strain with ligations (pACYC177+OmpA_alpha + deltaA and pACYC177+OmpA_omega + deltaA). <br>
2. Transformants plating on LB + kanamycin. </p>
2. Transformants plating on LB + kanamycin. </p>
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Oczyszczanie białek: A-alfa, Z-alfa i Z-omega
 
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Piotr
 
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Zwirowanie bakterii z 1l, sonikacja ich (protokół)
 
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zwirowanie sonikatu
 
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w przypadku z-alfa i z-omega odżucenie supernatantu, zawieszenie osadu w wodzi i ponowne zwirowanie i odżucenie wody i zawieszenie w wodzie
 
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w przypadku a-alfa zatrzymanie supernatantu odżucenie osadu
 
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Revision as of 23:23, 2 October 2008

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1. Transformation of E. coli TOP10 strain with ligations (pACYC177+OmpA_alpha + deltaA and pACYC177+OmpA_omega + deltaA).
2. Transformants plating on LB + kanamycin.



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