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Team:Hawaii/Milestone
From 2008.igem.org
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* Does RSF1010 replicate autonomously in PCC6803? (MR) | * Does RSF1010 replicate autonomously in PCC6803? (MR) | ||
* Compare RP4 sequence with OriT (MR) | * Compare RP4 sequence with OriT (MR) | ||
| + | * Learn how to extract plasmids from BioBrick kit filter paper -> test transform | ||
</onlyinclude> | </onlyinclude> | ||
Revision as of 05:55, 10 June 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Immediate
- 6/12/08 Final draft of proposal due
- 6/12/08 Design and order primers
- 6/12/08 Make list of BioBrick parts needed for Part B
- Culturing Protocol Optimization (KS)
- Does RSF1010 replicate autonomously in PCC6803? (MR)
- Compare RP4 sequence with OriT (MR)
- Learn how to extract plasmids from BioBrick kit filter paper -> test transform
Long Term
- 2008.06.27 Make final decision on Part B of our iGEM project
- Make gantt diagram for project
Completed Milestones
- 2008.05.xx figure out OD Reading operation: Beckman 640U spectrophotometer (AB/NW)
- 2008.05.24 Create seed stocks for test transformation (NW/MR)
- 2008.05.22 Describe, present [http://partsregistry.org/Part_Types part types]. 1. Norman: biobrick assembly, parts, 2. Margaret: devices, 3. Krystle: chassis & plasmids, 4. Grace: other
- 2008.06.01 Plasmid Transformation Plan/Protocol (GK/MR)
- 2008.06.02 Create competent DH5a E. coli stocks (GK/MR/NW)
- 2008.06.03-2008.06.04 Plasmid Transformation, document results (GK/NW)
- 2008.06.04 GK and KS are now autoclave trained
- Fluorometer found
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
