Team:Warsaw/Calendar-Main/24 July 2008

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<p>1. Separate transformant colonies (tranformation from previous day) inoculated to liquid LB with kanamycin.  
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<ol><li>Separate transformant colonies (tranformation of ligation of pACYC177+OmpA_alpha and omega_A from previous day) inoculated to liquid LB with kanamycin.  
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<h4>Antoni:</h4>
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<h3>Antoni:</h3>
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<ol><li>Preparation of <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#chemocompetent">chemocompetent</a> bacteria <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htmtop10">TOP10</a>.</li></ol>
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1. Preparation of <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#chemocompetent">chemocompetent</a> bacteria <i>E. coli</i> TOP10.<br>
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Revision as of 17:41, 5 October 2008

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  1. Separate transformant colonies (tranformation of ligation of pACYC177+OmpA_alpha and omega_A from previous day) inoculated to liquid LB with kanamycin.

Antoni:

  1. Preparation of chemocompetent bacteria E. coli TOP10.

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