Team:Warsaw/Calendar-Main/11 June 2008

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<html><h3>Michał L., Ewa, Marcin</h3>
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<html><h4>Michał L., Ewa, Marcin</h4>
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<p><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#chemotransform">Transformation</a> of <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> carrying <a href="https://2008.igem.org/Wiki/Team:Warsaw/vectors/pZC320">pZC320</a> with:
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<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#chemotransform">Transformation</a> of <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> carrying <a href="https://2008.igem.org/Wiki/Team:Warsaw/vectors/pZC320">pZC320</a> with:
<ul><li>pMPMT5,</li>  
<ul><li>pMPMT5,</li>  
<li>pMPMT5-AID,</li>  
<li>pMPMT5-AID,</li>  

Revision as of 19:51, 5 October 2008

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Michał L., Ewa, Marcin

  1. Transformation of E. coli TOP10 carrying pZC320 with:
    • pMPMT5,
    • pMPMT5-AID,
    • pMPMT5-AIDT7,
    • or pMPMT5-AiD+T7.

    Plating transformants on 'TAXI' (LB + Tetracycline + IPTG + Ampicillin 30 μg/mL + X-Gal).

    Rifampicin test of transformants.

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