Team:Warsaw/Calendar-Main/11 June 2008

From 2008.igem.org

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<li>pMPMT5-AIDT7, </li>
<li>pMPMT5-AIDT7, </li>
<li>or pMPMT5-AiD+T7.</li></ul>
<li>or pMPMT5-AiD+T7.</li></ul>
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<p>Plating transformants on 'TAXI' (LB + Tetracycline + IPTG + Ampicillin 30 μg/mL + X-Gal).</p>
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<li>Plating transformants on 'TAXI' (LB + Tetracycline + IPTG + Ampicillin 30 μg/mL + X-Gal).</li>
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<p><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#rif">Rifampicin test</a> of transformants.</p>
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<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#rif">Rifampicin test</a> of transformants.</li>
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Michał L., Ewa, Marcin

  1. Transformation of E. coli TOP10 carrying pZC320 with:
    • pMPMT5,
    • pMPMT5-AID,
    • pMPMT5-AIDT7,
    • or pMPMT5-AiD+T7.
  2. Plating transformants on 'TAXI' (LB + Tetracycline + IPTG + Ampicillin 30 μg/mL + X-Gal).
  3. Rifampicin test of transformants.

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