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Team:Illinois/Antibody GPCR Fusion Notebook
From 2008.igem.org
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| - | + | == 22nd July == | |
* Yeast obtained from Dr. Zhao | * Yeast obtained from Dr. Zhao | ||
| - | + | == 24th July == | |
* Prepared liquid culture for DNA extraction | * Prepared liquid culture for DNA extraction | ||
* Made 1M Tris. Cl pH 8.0 | * Made 1M Tris. Cl pH 8.0 | ||
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| - | + | == 22nd August == | |
* Attempted DNA extraction | * Attempted DNA extraction | ||
** Result: Failed | ** Result: Failed | ||
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| - | + | == 25th August == | |
* Prepared overnight culture for DNA extraction (3:27pm) | * Prepared overnight culture for DNA extraction (3:27pm) | ||
| - | + | == 26th August == | |
* Attempted DNA extraction | * Attempted DNA extraction | ||
* Prepped overnight culture | * Prepped overnight culture | ||
| - | + | == 27th August == | |
* Performed PCR (FILL IN PCR TABLE) | * Performed PCR (FILL IN PCR TABLE) | ||
* Prepped 3 overnight cultures | * Prepped 3 overnight cultures | ||
| - | + | == 28th August == | |
* Extracted DNA from 4 cultures | * Extracted DNA from 4 cultures | ||
* Ran gel of PCR products (1.5%, 200V) | * Ran gel of PCR products (1.5%, 200V) | ||
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Revision as of 20:17, 7 October 2008
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== 22nd July ==
- Yeast obtained from Dr. Zhao
== 24th July ==
- Prepared liquid culture for DNA extraction
- Made 1M Tris. Cl pH 8.0
- Made 4M ammonium acetate
== 22nd August ==
- Attempted DNA extraction
- Result: Failed
- Obtained more yeast from Dr. Zhao
== 25th August ==
- Prepared overnight culture for DNA extraction (3:27pm)
== 26th August ==
- Attempted DNA extraction
- Prepped overnight culture
== 27th August ==
- Performed PCR (FILL IN PCR TABLE)
- Prepped 3 overnight cultures
== 28th August ==
- Extracted DNA from 4 cultures
- Ran gel of PCR products (1.5%, 200V)
- Result: No bands present
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