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Team:Illinois/Antibody GPCR Fusion Notebook
From 2008.igem.org
(Difference between revisions)
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<!--PLACE TEXT BELOW HERE --> | <!--PLACE TEXT BELOW HERE --> | ||
| - | + | !-- == 22nd July == | |
* Yeast obtained from Dr. Zhao | * Yeast obtained from Dr. Zhao | ||
| - | + | !-- == 24th July == | |
* Prepared liquid culture for DNA extraction | * Prepared liquid culture for DNA extraction | ||
* Made 1M Tris. Cl pH 8.0 | * Made 1M Tris. Cl pH 8.0 | ||
| Line 13: | Line 13: | ||
| - | + | !-- == 22nd August == | |
* Attempted DNA extraction | * Attempted DNA extraction | ||
** Result: Failed | ** Result: Failed | ||
| Line 19: | Line 19: | ||
| - | + | !-- == 25th August == | |
* Prepared overnight culture for DNA extraction (3:27pm) | * Prepared overnight culture for DNA extraction (3:27pm) | ||
| - | + | !-- == 26th August == | |
* Attempted DNA extraction | * Attempted DNA extraction | ||
* Prepped overnight culture | * Prepped overnight culture | ||
| - | + | !-- == 27th August == | |
* Performed PCR (FILL IN PCR TABLE) | * Performed PCR (FILL IN PCR TABLE) | ||
* Prepped 3 overnight cultures | * Prepped 3 overnight cultures | ||
| - | + | !-- == 28th August == | |
* Extracted DNA from 4 cultures | * Extracted DNA from 4 cultures | ||
* Ran gel of PCR products (1.5%, 200V) | * Ran gel of PCR products (1.5%, 200V) | ||
| Line 39: | Line 39: | ||
| + | !-- == 2nd September == | ||
| + | * FILL IN PCR TABLE | ||
| + | |||
| + | !-- == 3rd September == | ||
| + | * Ran gel | ||
| + | ** Ladder lane 7 | ||
| + | ** Sample 7 spilled | ||
| + | ** 1% agarose | ||
| + | *** Too high | ||
| + | ** 120V | ||
| + | *** Too low | ||
| + | ** 50 minutes | ||
Revision as of 20:25, 7 October 2008
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!-- == 22nd July ==
- Yeast obtained from Dr. Zhao
!-- == 24th July ==
- Prepared liquid culture for DNA extraction
- Made 1M Tris. Cl pH 8.0
- Made 4M ammonium acetate
!-- == 22nd August ==
- Attempted DNA extraction
- Result: Failed
- Obtained more yeast from Dr. Zhao
!-- == 25th August ==
- Prepared overnight culture for DNA extraction (3:27pm)
!-- == 26th August ==
- Attempted DNA extraction
- Prepped overnight culture
!-- == 27th August ==
- Performed PCR (FILL IN PCR TABLE)
- Prepped 3 overnight cultures
!-- == 28th August ==
- Extracted DNA from 4 cultures
- Ran gel of PCR products (1.5%, 200V)
- Result: No bands present
!-- == 2nd September ==
- FILL IN PCR TABLE
!-- == 3rd September ==
- Ran gel
- Ladder lane 7
- Sample 7 spilled
- 1% agarose
- Too high
- 120V
- Too low
- 50 minutes
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