Team:Warsaw/Calendar-Main/20 June 2008

From 2008.igem.org

(Difference between revisions)
Line 30: Line 30:
<ol>
<ol>
<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation</a> of plasmids from cultures inoculated on previous day.</li>  
<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation</a> of plasmids from cultures inoculated on previous day.</li>  
-
 
+
<li>Control <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">Digest</a> of isolated plasmids with PstI.</li>
 +
<li>Gel electrophoresis (no proper colonies founded).</li></ol>
</html>
</html>

Revision as of 14:56, 8 October 2008

Gallery Bricks Notebook Team Project Home


Previous day
return to main notebook page
Previous entry
Interactive list of topics
next notebook entry

 


Michał L., Ewa, Marcin:

PCR
ProductTemplatePrimersProduct length
linker-ApDRIVE-TapTagAL+link10+homo2 and AP+NotI 470 bp
alpha-linkerpUC19 AlphaL+SacI and AlphaP+link10+homo2600 bp
omega-linkerpUC19OmegaL+SacI and OmegaP+link10+homo2400 bp

Universal PCR program for A, alpha and omega

TemperatureTime
94°C4:00
94°C0:3028 cycles
50°C0:45
72°C2:00
72°C10:00
4°Cinfinite

Polymerase: Pfu-Turbo

Preparation of constructs with OmpA protein fusions

Michał K.:

  1. Isolation of plasmids from cultures inoculated on previous day.
  2. Control Digest of isolated plasmids with PstI.
  3. Gel electrophoresis (no proper colonies founded).

Retrieved from "http://2008.igem.org/Team:Warsaw/Calendar-Main/20_June_2008"