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Team:Hawaii/Notebook/2008-10- 8
From 2008.igem.org
(Difference between revisions)
(New page: {{Team:Hawaii/Header}} = Things we did today = == Wetlab work == ===Triparental conjugation (cont.)=== :<strong> Grace</strong> :* PCR'd BBpRL cultures to ensure that plasmid was retaine...) |
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===iGEM title/abstract=== | ===iGEM title/abstract=== | ||
| - | :<strong>Grace & Margaret<strong> | + | :<strong>Grace & Margaret</strong> |
:* Revised abstract | :* Revised abstract | ||
:* Sent in abstract, title, track selection to iGEM HQ | :* Sent in abstract, title, track selection to iGEM HQ | ||
| + | |||
= Discussion = | = Discussion = | ||
Revision as of 02:04, 9 October 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Triparental conjugation (cont.)
- Grace
- PCR'd BBpRL cultures to ensure that plasmid was retained
- Plasmid was not retained (grown in SOB w/o selection = no reason to retain plasmid)
- PCR'd BBpRL colonies #1 and 18 from plate to ensure plasmid present
- Regrew RP1, BBpRL harboring cultures in 20ml SOB with selection (Kan50 and Sp100 respectively) and incubated at 37C overnight, with shaking (rpm = 250)
Verification of transformants
- Grace
| Construct | Colonies |
|---|---|
| nir + rgt#1 | 1 |
| plac + rgt#1 | 0 |
| nr + sg | 12 |
| pr + sg | 74 |
| nr + pgt | 5 |
| pr + pgt | 19 |
| pSB1A3 (control) | 1 |
| no DNA | 0 |
- Colony PCR of transformants
- Restreaked nrsg #6, prpgt #5, 7, 11
Construction of secretion device (cont.)
- Grace
- PCR:
- J33207
- nir+rbs
- nir
- rgt#1
- Overnight RE digest:
- HindIII/BamHI:
- J33207
- EcoRI/SpeI:
- nir
- nir+rbs
- XbaI/PstI:
- rgt#1
Drylab work
iGEM title/abstract
- Grace & Margaret
- Revised abstract
- Sent in abstract, title, track selection to iGEM HQ
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
