Team:Warsaw/Calendar-Main/1 July 2008

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<h3>Preparation of constructs with OmpA protein fusions<br>Paweł</h3>
<h3>Preparation of constructs with OmpA protein fusions<br>Paweł</h3>
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<p><ol><li> Overnight <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#digest">digest</a> of pET15b plasmid with NdeI and BamHI, <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#removing">dephosphorylation with CIAP</a>.</li></ol></p>
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<p><ol>
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<li> Isolation of pET15b plasmid.</li>
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<li> Overnight <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#digest">digest</a> of pET15b plasmid with NdeI and BamHI, <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#removing">dephosphorylation with CIAP</a>.</li></ol></p>
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Preparation of constructs with OmpA protein fusions
Paweł

  1. Isolation of pET15b plasmid.
  2. Overnight digest of pET15b plasmid with NdeI and BamHI, dephosphorylation with CIAP.

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