Revision as of 09:25, 11 June 2008

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The protocols we used

(estimated time: 1 hour and 30 min)

Materials needed:

Add the proper amount of 10X buffer to a final concentration of 1X (e.g. 2 )
Cut paper spots using scalpel and tweezers (or punch tool, following provided instructions); if you use scalpel and tweezers, try to cut pieces of about the same dimension of the punch tool.
Put the cut paper into the 0.5 ml tube.
Clean scalpel and tweezers (or punch tool) with water and ethanol every time you cut a spot; be careful to dry your tools correctly, especially if you use punch tool, which needs much more time to dry than scalpel/tweezers.
Incubate at 42°C for 20 min.
Vortex and spin down.

@@ Line 27: / Line 27: @@
-<h1>Plasmid resuspension from IGEM paper spots</h1>
+<h1>Antarctic Phosphatase</h1>
-''(estimated time: 25 min + 5 min for every part if you use scalpel/tweezers or + 15 min for every part if you use punch tool)''
+''(estimated time: 1 hour and 30 min)''
 <br>
 <br>
 '''Materials needed:'''
-*'''99% ethanol'''
+*'''NEB Antarctic Phosphatase'''
-*'''0.5 ml tubes'''
+*'''10X NEB Antarctic Phosphatase buffer'''
+*'''Cut and gel-extracted vector'''
 <br>
-*Put 10 µl of pre-warmed TE into a 0.5 ml tube.
+*Add the proper amount of 10X buffer to a final concentration of 1X (e.g. 2 )
 *Cut paper spots using scalpel and tweezers (or punch tool, following provided instructions); if you use scalpel and tweezers, try to cut pieces of about the same dimension of the punch tool.
 *Put the cut paper into the 0.5 ml tube.