Team:Hawaii/Notebook/2008-10-10

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(New page: {{Team:Hawaii/Header}} = Things we did today = == Wetlab work == ===verification of Transformants=== :<strong> Grace</strong> [[Image:101008colonypcr.jpg|right|thumb|500px|EtBr stained 2%...)
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Revision as of 02:30, 11 October 2008

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Things we did today

Wetlab work

verification of Transformants

Grace
File:101008colonypcr.jpg
EtBr stained 2% agarose gel ran at 95V for 1 hour. Two microliters of PCR product were loaded into each well.
Construct Colonies
nir+rbs+GFP+tt#1 100
plac+rbs+GFP+tt#1 3
nir+rbs+slr1+GFPf 51
nir+rbs+pilA+GFPf+tt 24
BBpRL1383a lawn
(-) no DNA 0
  • Colony PCR of transformants


Triparental Conjugation

Grace
  • Began triparental conjugation between E. coli containing RP1 and BBpRL1383a -1 or -18 and Synechocystis
  • RP1 OD600=0.6341
  • BBpRL1383-a OD600=61.25
  • BBpRL1383a-18 OD600=0.4884
  • Synechocystis OD700=0.8629, OD730=0.7337
  • Placed plates in SC 37C CO2 incubator until Monday


Discussion

Quote of the Day

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson


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